Functional evaluation of chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas

J. Y. Kim, J. I. Lee, J. H. Jeong, Y. J. Park, S. J. Kim, Y. H. Fang, Y. Cho, K. H. Huh, D. J. Joo, M. S. Kim, YuSeun Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In islet transplantation, encapsulation of immunoisolated islets may provide a way to protect the graft from immune attacks with no immunosuppression. To develop an immunodelusive immunoisolated bioartificial pancreas (BAP), chondrocyte sheets were prepared by cell sheet engineering. We made an immunoisolated BAP encapsulated with rodent-derived chondrocyte sheets and then evaluated its function. Sprague-Dawley rats were used as the source of auricular cartilage and chondrocytes were maintained and expanded by passages. Lewis rats were prepared for islet isolation. A 3-dimensional chondrocyte sheeting immunodelusive immunoisolated BAP (CSI-BAP) was created by multi-layering and unifying the chondrocyte sheets. Subsequently, islets were embedded between each multi-layer sheet. To evaluate the function of the CSI-BAP, a glucose challenge test was performed and secretion of insulin in the culture medium was measured by an enzyme-linked immunosorbent assay. When observed by phase-contrast microscopy, the CSI-BAP maintained close connections between chondrocyte sheets. Islets in the CSI-BAP maintained viability at day 10 and showed good insulin secretion, as revealed by a prompt reaction to increased concentrations of glucose at days 5 and 10. In long-term culture, the CSI-BAP maintained its ability to secrete insulin for 8 weeks. This BAP technology could be an important tool for successful islet transplantation without immunosuppressive drugs.

Original languageEnglish
Pages (from-to)903-906
Number of pages4
JournalTransplantation Proceedings
Volume42
Issue number3
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Chondrocytes
Pancreas
Islets of Langerhans Transplantation
Insulin
Ear Cartilage
Cell Engineering
Phase-Contrast Microscopy
Glucose
Immunosuppressive Agents
Immunosuppression
Sprague Dawley Rats
Culture Media
Rodentia
Enzyme-Linked Immunosorbent Assay
Technology
Transplants

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Kim, J. Y. ; Lee, J. I. ; Jeong, J. H. ; Park, Y. J. ; Kim, S. J. ; Fang, Y. H. ; Cho, Y. ; Huh, K. H. ; Joo, D. J. ; Kim, M. S. ; Kim, YuSeun. / Functional evaluation of chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas. In: Transplantation Proceedings. 2010 ; Vol. 42, No. 3. pp. 903-906.
@article{a34d1eef5a864ebfa6b6d4028cf37a7d,
title = "Functional evaluation of chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas",
abstract = "In islet transplantation, encapsulation of immunoisolated islets may provide a way to protect the graft from immune attacks with no immunosuppression. To develop an immunodelusive immunoisolated bioartificial pancreas (BAP), chondrocyte sheets were prepared by cell sheet engineering. We made an immunoisolated BAP encapsulated with rodent-derived chondrocyte sheets and then evaluated its function. Sprague-Dawley rats were used as the source of auricular cartilage and chondrocytes were maintained and expanded by passages. Lewis rats were prepared for islet isolation. A 3-dimensional chondrocyte sheeting immunodelusive immunoisolated BAP (CSI-BAP) was created by multi-layering and unifying the chondrocyte sheets. Subsequently, islets were embedded between each multi-layer sheet. To evaluate the function of the CSI-BAP, a glucose challenge test was performed and secretion of insulin in the culture medium was measured by an enzyme-linked immunosorbent assay. When observed by phase-contrast microscopy, the CSI-BAP maintained close connections between chondrocyte sheets. Islets in the CSI-BAP maintained viability at day 10 and showed good insulin secretion, as revealed by a prompt reaction to increased concentrations of glucose at days 5 and 10. In long-term culture, the CSI-BAP maintained its ability to secrete insulin for 8 weeks. This BAP technology could be an important tool for successful islet transplantation without immunosuppressive drugs.",
author = "Kim, {J. Y.} and Lee, {J. I.} and Jeong, {J. H.} and Park, {Y. J.} and Kim, {S. J.} and Fang, {Y. H.} and Y. Cho and Huh, {K. H.} and Joo, {D. J.} and Kim, {M. S.} and YuSeun Kim",
year = "2010",
month = "12",
day = "1",
doi = "10.1016/j.transproceed.2010.02.043",
language = "English",
volume = "42",
pages = "903--906",
journal = "Transplantation Proceedings",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "3",

}

Kim, JY, Lee, JI, Jeong, JH, Park, YJ, Kim, SJ, Fang, YH, Cho, Y, Huh, KH, Joo, DJ, Kim, MS & Kim, Y 2010, 'Functional evaluation of chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas', Transplantation Proceedings, vol. 42, no. 3, pp. 903-906. https://doi.org/10.1016/j.transproceed.2010.02.043

Functional evaluation of chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas. / Kim, J. Y.; Lee, J. I.; Jeong, J. H.; Park, Y. J.; Kim, S. J.; Fang, Y. H.; Cho, Y.; Huh, K. H.; Joo, D. J.; Kim, M. S.; Kim, YuSeun.

In: Transplantation Proceedings, Vol. 42, No. 3, 01.12.2010, p. 903-906.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Functional evaluation of chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas

AU - Kim, J. Y.

AU - Lee, J. I.

AU - Jeong, J. H.

AU - Park, Y. J.

AU - Kim, S. J.

AU - Fang, Y. H.

AU - Cho, Y.

AU - Huh, K. H.

AU - Joo, D. J.

AU - Kim, M. S.

AU - Kim, YuSeun

PY - 2010/12/1

Y1 - 2010/12/1

N2 - In islet transplantation, encapsulation of immunoisolated islets may provide a way to protect the graft from immune attacks with no immunosuppression. To develop an immunodelusive immunoisolated bioartificial pancreas (BAP), chondrocyte sheets were prepared by cell sheet engineering. We made an immunoisolated BAP encapsulated with rodent-derived chondrocyte sheets and then evaluated its function. Sprague-Dawley rats were used as the source of auricular cartilage and chondrocytes were maintained and expanded by passages. Lewis rats were prepared for islet isolation. A 3-dimensional chondrocyte sheeting immunodelusive immunoisolated BAP (CSI-BAP) was created by multi-layering and unifying the chondrocyte sheets. Subsequently, islets were embedded between each multi-layer sheet. To evaluate the function of the CSI-BAP, a glucose challenge test was performed and secretion of insulin in the culture medium was measured by an enzyme-linked immunosorbent assay. When observed by phase-contrast microscopy, the CSI-BAP maintained close connections between chondrocyte sheets. Islets in the CSI-BAP maintained viability at day 10 and showed good insulin secretion, as revealed by a prompt reaction to increased concentrations of glucose at days 5 and 10. In long-term culture, the CSI-BAP maintained its ability to secrete insulin for 8 weeks. This BAP technology could be an important tool for successful islet transplantation without immunosuppressive drugs.

AB - In islet transplantation, encapsulation of immunoisolated islets may provide a way to protect the graft from immune attacks with no immunosuppression. To develop an immunodelusive immunoisolated bioartificial pancreas (BAP), chondrocyte sheets were prepared by cell sheet engineering. We made an immunoisolated BAP encapsulated with rodent-derived chondrocyte sheets and then evaluated its function. Sprague-Dawley rats were used as the source of auricular cartilage and chondrocytes were maintained and expanded by passages. Lewis rats were prepared for islet isolation. A 3-dimensional chondrocyte sheeting immunodelusive immunoisolated BAP (CSI-BAP) was created by multi-layering and unifying the chondrocyte sheets. Subsequently, islets were embedded between each multi-layer sheet. To evaluate the function of the CSI-BAP, a glucose challenge test was performed and secretion of insulin in the culture medium was measured by an enzyme-linked immunosorbent assay. When observed by phase-contrast microscopy, the CSI-BAP maintained close connections between chondrocyte sheets. Islets in the CSI-BAP maintained viability at day 10 and showed good insulin secretion, as revealed by a prompt reaction to increased concentrations of glucose at days 5 and 10. In long-term culture, the CSI-BAP maintained its ability to secrete insulin for 8 weeks. This BAP technology could be an important tool for successful islet transplantation without immunosuppressive drugs.

UR - http://www.scopus.com/inward/record.url?scp=77955475287&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955475287&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2010.02.043

DO - 10.1016/j.transproceed.2010.02.043

M3 - Article

C2 - 20430200

AN - SCOPUS:77955475287

VL - 42

SP - 903

EP - 906

JO - Transplantation Proceedings

JF - Transplantation Proceedings

SN - 0041-1345

IS - 3

ER -