Functional expression of P2Y receptors in WERI-Rb1 retinoblastoma cells

Na Hyun Kim, Kyusang Park, Joon Hyung Sohn, Byung Il Yeh, Chang Mann Ko, Indeok Kong

Research output: Contribution to journalArticle

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Abstract

P2Y receptors are metabotropic G-protein-coupled receptors, which are involved in many important biologic functions in the central nervous system including retina. Subtypes of P2Y receptors in retinal tissue vary according to the species and the cell types. We examined the molecular and pharmacologic profiles of P2Y purinoceptors in retinoblastoma cell, which has not been identified yet. To achieve this goal, we used Ca 2+ imaging technique and western blot analysis in WERI-Rb-1 cell, a human retinoblastoma cell line. ATP (10 μM) elicited strong but transient [Ca 2+ ] i increase in a concentration-dependent manner from more than 80% of the WERI-Rb-1 cells (n=46). Orders of potency of P2Y agonists in evoking [Ca 2+ ] i transients were 2MeS-ATP>ATP≫UTP= αβ -MeATP, which was compatible with the subclass of P2Y 1 receptor. The [Ca 2+ ] i transients evoked by applications of 2MeS-ATP and/or ATP were also profoundly suppressed in the presence of P2Y 1 selective blocker (MRS 2179; 30 μM). P2Y 1 receptor expression in WERI-Rb-1 cells was also identified by using western blot. Taken together, P2Y 1 receptor is mainly expressed in a retinoblastoma cell, which elicits Ca 2+ release from internal Ca storage sites via the phospholipase C-mediated pathway. P2Y 1 receptor activation in retino-blastoma cell could be a useful model to investigate the role of purinergic [Ca 2+ ] i signaling in neural tissue as well as to find a novel therapeutic target to this lethal cancer.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalKorean Journal of Physiology and Pharmacology
Volume15
Issue number1
DOIs
Publication statusPublished - 2011 Feb 1

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Retinoblastoma
Adenosine Triphosphate
Purinergic P2Y Receptors
Western Blotting
Type C Phospholipases
G-Protein-Coupled Receptors
Retina
Central Nervous System
Cell Line
Neoplasms

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology

Cite this

Kim, Na Hyun ; Park, Kyusang ; Sohn, Joon Hyung ; Yeh, Byung Il ; Ko, Chang Mann ; Kong, Indeok. / Functional expression of P2Y receptors in WERI-Rb1 retinoblastoma cells. In: Korean Journal of Physiology and Pharmacology. 2011 ; Vol. 15, No. 1. pp. 61-66.
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Functional expression of P2Y receptors in WERI-Rb1 retinoblastoma cells. / Kim, Na Hyun; Park, Kyusang; Sohn, Joon Hyung; Yeh, Byung Il; Ko, Chang Mann; Kong, Indeok.

In: Korean Journal of Physiology and Pharmacology, Vol. 15, No. 1, 01.02.2011, p. 61-66.

Research output: Contribution to journalArticle

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