Functional identification of p-cumate operons in the terpene-degrading Rhodococcus sp. strain T104

Hyun Won Bae, Dockyu Kim, Ki Young Choi, Na Ra Kwon, Jong Chan Chae, Gerben J. Zylstra, Sung Cheol Koh, Choong Hwan Lee, Eungbin Kim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We identified a p-cumate degrading gene cluster (designated cmt) with a novel organization in our genomic studies of the terpene-degrading Rhodococcus sp. strain T104. The mutant strain SN140, isolated for the inability to grow on p-cumate, accumulates 2,3-dihydroxy-p-cumate as identified by liquid chromatography-mass spectrometry and 300 MHz proton nuclear magnetic resonance spectroscopy. Reverse transcriptase polymerase chain reaction experiments showed that the cmt genes are operonic and induced specifically by growth on p-cumate. This report is the first example of identifying the genes for p-cumate degradation in a gram-positive organism based on functional data.

Original languageEnglish
Pages (from-to)54-59
Number of pages6
JournalFEMS Microbiology Letters
Volume266
Issue number1
DOIs
Publication statusPublished - 2007 Jan

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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