Functional identification of p-cumate operons in the terpene-degrading Rhodococcus sp. strain T104

Hyun Won Bae, Dockyu Kim, Ki Young Choi, Na Ra Kwon, Jong Chan Chae, Gerben J. Zylstra, Sung Cheol Koh, Choong Hwan Lee, Eungbin Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We identified a p-cumate degrading gene cluster (designated cmt) with a novel organization in our genomic studies of the terpene-degrading Rhodococcus sp. strain T104. The mutant strain SN140, isolated for the inability to grow on p-cumate, accumulates 2,3-dihydroxy-p-cumate as identified by liquid chromatography-mass spectrometry and 300 MHz proton nuclear magnetic resonance spectroscopy. Reverse transcriptase polymerase chain reaction experiments showed that the cmt genes are operonic and induced specifically by growth on p-cumate. This report is the first example of identifying the genes for p-cumate degradation in a gram-positive organism based on functional data.

Original languageEnglish
Pages (from-to)54-59
Number of pages6
JournalFEMS Microbiology Letters
Volume266
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Rhodococcus
Terpenes
Operon
Multigene Family
Reverse Transcriptase Polymerase Chain Reaction
Liquid Chromatography
Genes
Mass Spectrometry
Magnetic Resonance Spectroscopy
cumic acid
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Bae, Hyun Won ; Kim, Dockyu ; Choi, Ki Young ; Kwon, Na Ra ; Chae, Jong Chan ; Zylstra, Gerben J. ; Koh, Sung Cheol ; Lee, Choong Hwan ; Kim, Eungbin. / Functional identification of p-cumate operons in the terpene-degrading Rhodococcus sp. strain T104. In: FEMS Microbiology Letters. 2007 ; Vol. 266, No. 1. pp. 54-59.
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Functional identification of p-cumate operons in the terpene-degrading Rhodococcus sp. strain T104. / Bae, Hyun Won; Kim, Dockyu; Choi, Ki Young; Kwon, Na Ra; Chae, Jong Chan; Zylstra, Gerben J.; Koh, Sung Cheol; Lee, Choong Hwan; Kim, Eungbin.

In: FEMS Microbiology Letters, Vol. 266, No. 1, 01.01.2007, p. 54-59.

Research output: Contribution to journalArticle

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AU - Bae, Hyun Won

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AU - Chae, Jong Chan

AU - Zylstra, Gerben J.

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