Functional improvement of hemostatic dressing by addition of recombinant batroxobin

Gyeung Mi Seon, Mi Hee Lee, Byeong Ju Kwon, Min Sung Kim, Min Ah Koo, Dohyun Kim, Young Seomun, Jong Tak Kim, Jongchul Park

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although a number of natural materials have been used as hemostatic agents, many substances do not act quickly enough. Here, we created a novel dressings using collagen and chitosan with recombinant batroxobin (r-Bat) to promote faster and more effective hemostasis. We hypothesized that r-Bat would promote synergetic blood coagulation because it contains a blood coagulation active site different than those of collagen and chitosan. Our results suggest that each substances can maintain hemostatic properties while in the mixed dressings and that our novel hemostatic dressings promotes potent control of bleeding, as demonstrated by a whole blood assay and rat hemorrhage model. In a rat femoral artery model, the scaffold with a high r-Bat concentration more rapidly controlled excessive bleeding. This novel dressings has enormous possible for rapidly controlling bleeding and it improves upon the effect of collagen and chitosan used alone. Our novel r-Bat dressings is a possible candidate for improving preoperative care and displays promising properties as an absorbable agent in hemostasis. Statement of Significance Despite the excellent hemostatic properties of collagen and chitosan pads, they reported to brittle behavior and lack sufficient hemostatic effect within relevant time. Therefore, we created a novel pad using collagen and chitosan with recombinant batroxobin (r-Bat). r-Bat acts as a thrombin-like enzyme in the coagulation cascade. Specifically, r-Bat, in contrast to thrombin, only splits fibrinopeptide A off and does not influence other hemostatic factors or cells, which makes it clinically useful as a stable hemostatic agent. Also the materials in the pad have synergetic effect because they have different hemostatic mechanisms in the coagulation cascade. This report propose the novel hemostatic pad isreasonable that a great potential for excessive bleeding injury and improve effects of natural substance hemostatic pad.

Original languageEnglish
Pages (from-to)175-185
Number of pages11
JournalActa Biomaterialia
Volume48
DOIs
Publication statusPublished - 2017 Jan 15

Fingerprint

Batroxobin
Hemostatics
Bandages
Chitosan
Collagen
Coagulation
Blood
Rats
Hemorrhage
Blood Coagulation
Hemostasis
Scaffolds
Thrombin
Assays
Enzymes
Fibrinopeptide A
Preoperative Care
Femoral Artery
Catalytic Domain

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Seon, Gyeung Mi ; Lee, Mi Hee ; Kwon, Byeong Ju ; Kim, Min Sung ; Koo, Min Ah ; Kim, Dohyun ; Seomun, Young ; Kim, Jong Tak ; Park, Jongchul. / Functional improvement of hemostatic dressing by addition of recombinant batroxobin. In: Acta Biomaterialia. 2017 ; Vol. 48. pp. 175-185.
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Seon, GM, Lee, MH, Kwon, BJ, Kim, MS, Koo, MA, Kim, D, Seomun, Y, Kim, JT & Park, J 2017, 'Functional improvement of hemostatic dressing by addition of recombinant batroxobin', Acta Biomaterialia, vol. 48, pp. 175-185. https://doi.org/10.1016/j.actbio.2016.10.024

Functional improvement of hemostatic dressing by addition of recombinant batroxobin. / Seon, Gyeung Mi; Lee, Mi Hee; Kwon, Byeong Ju; Kim, Min Sung; Koo, Min Ah; Kim, Dohyun; Seomun, Young; Kim, Jong Tak; Park, Jongchul.

In: Acta Biomaterialia, Vol. 48, 15.01.2017, p. 175-185.

Research output: Contribution to journalArticle

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AU - Seon, Gyeung Mi

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