Functional inhibition of fatty acid binding protein 4 ameliorates impaired ciliogenesis in GCs

Yooju Jung, Sung Min Cho, Sungsoo Kim, Jae Ho Cheong, Ho Jeong Kwon

Research output: Contribution to journalArticlepeer-review

Abstract

Ciliogenesis is often impaired in some cancer cells, leading to acceleration of cancer phenotypes such as cell migration and proliferation. From the investigation of primary cilia of 16 gastric cancer cells (GCs), we found that GCs could be grouped into four primary cilia (PC)–positive GCs and 12 PC-negative GCs. The proliferation of the PC-positive GCs was lower than that of PC-negative GCs. To explore the role of fatty acid binding protein 4 (FABP4), which is a known oncogenic factor, in ciliogenesis, FABP4 expression and function were inhibited by transfection of cells with short interfering RNA targeting FABP4 (siFABP4) or FABP4 inhibitor treatment. Notably, the proliferation and migration of the cilia-forming GCs was effectively suppressed by inhibition of FABP4. In addition, the primary cilia in GCs were restored by a factor greater than two, suggesting a negative role of FABP4 in ciliogenesis in these GCs and FABP4 as a potential anticancer target.

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume539
DOIs
Publication statusPublished - 2021 Feb 5

Bibliographical note

Funding Information:
This work was partly supported by grants from the National Research Foundation of Korea , funded by the Korean government ( MSIP ; 2015M3A9B6027818 , 2015K1A1A202836523 ) and by the Brain Korea 21 Plus Project and ICONS (Institute of Convergence Science), Yonsei University .

Publisher Copyright:
© 2020 Elsevier Inc.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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