Functional manipulation of dendritic cells by photoswitchable generation of intracellular reactive oxygen species

Taek Chin Cheong, Eon Pil Shin, Eun Kyung Kwon, Ji Hye Choi, Kang Kyun Wang, Prashant Sharma, Kyong Hoon Choi, Jin Muk Lim, Hong Gee Kim, Keunhee Oh, Ju Hong Jeon, Insuk So, In Gyu Kim, Myung Sik Choi, Young Keun Kim, Seung Yong Seong, Yong Rok Kim, Nam Hyuk Cho

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) play an important role in cellular signaling as second messengers. However, studying the role of ROS in physiological redox signaling has been hampered by technical difficulties in controlling their generation within cells. Here, we utilize two inert components, a photosensitizer and light, to finely manipulate the generation of intracellular ROS and examine their specific role in activating dendritic cells (DCs). Photoswitchable generation of intracellular ROS rapidly induced cytosolic mobilization of Ca2+, differential activation of mitogen-activated protein kinases, and nuclear translocation of NF-κB. Moreover, a transient intracellular ROS surge could activate immature DCs to mature and potently enhance migration in vitro and in vivo. Finally, we observed that intracellular ROS-stimulated DCs enhanced antigen specific T-cell responses in vitro and in vivo, which led to delayed tumor growth and prolonged survival of tumor-bearing mice when immunized with a specific tumor antigen. Therefore, a transient intracellular ROS surge alone, if properly manipulated, can cause immature DCs to differentiate into a motile state and mature forms that are sufficient to initiate adaptive T cell responses in vivo.

Original languageEnglish
Pages (from-to)757-765
Number of pages9
JournalACS Chemical Biology
Volume10
Issue number3
DOIs
Publication statusPublished - 2015 Mar 20

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Dendritic Cells
Reactive Oxygen Species
T-cells
Tumors
Bearings (structural)
Cell signaling
T-Lymphocytes
Photosensitizing Agents
Second Messenger Systems
Neoplasm Antigens
Mitogen-Activated Protein Kinases
Oxidation-Reduction
Neoplasms
Chemical activation
Light
Antigens
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine

Cite this

Cheong, T. C., Shin, E. P., Kwon, E. K., Choi, J. H., Wang, K. K., Sharma, P., ... Cho, N. H. (2015). Functional manipulation of dendritic cells by photoswitchable generation of intracellular reactive oxygen species. ACS Chemical Biology, 10(3), 757-765. https://doi.org/10.1021/cb5009124
Cheong, Taek Chin ; Shin, Eon Pil ; Kwon, Eun Kyung ; Choi, Ji Hye ; Wang, Kang Kyun ; Sharma, Prashant ; Choi, Kyong Hoon ; Lim, Jin Muk ; Kim, Hong Gee ; Oh, Keunhee ; Jeon, Ju Hong ; So, Insuk ; Kim, In Gyu ; Choi, Myung Sik ; Kim, Young Keun ; Seong, Seung Yong ; Kim, Yong Rok ; Cho, Nam Hyuk. / Functional manipulation of dendritic cells by photoswitchable generation of intracellular reactive oxygen species. In: ACS Chemical Biology. 2015 ; Vol. 10, No. 3. pp. 757-765.
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abstract = "Reactive oxygen species (ROS) play an important role in cellular signaling as second messengers. However, studying the role of ROS in physiological redox signaling has been hampered by technical difficulties in controlling their generation within cells. Here, we utilize two inert components, a photosensitizer and light, to finely manipulate the generation of intracellular ROS and examine their specific role in activating dendritic cells (DCs). Photoswitchable generation of intracellular ROS rapidly induced cytosolic mobilization of Ca2+, differential activation of mitogen-activated protein kinases, and nuclear translocation of NF-κB. Moreover, a transient intracellular ROS surge could activate immature DCs to mature and potently enhance migration in vitro and in vivo. Finally, we observed that intracellular ROS-stimulated DCs enhanced antigen specific T-cell responses in vitro and in vivo, which led to delayed tumor growth and prolonged survival of tumor-bearing mice when immunized with a specific tumor antigen. Therefore, a transient intracellular ROS surge alone, if properly manipulated, can cause immature DCs to differentiate into a motile state and mature forms that are sufficient to initiate adaptive T cell responses in vivo.",
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Cheong, TC, Shin, EP, Kwon, EK, Choi, JH, Wang, KK, Sharma, P, Choi, KH, Lim, JM, Kim, HG, Oh, K, Jeon, JH, So, I, Kim, IG, Choi, MS, Kim, YK, Seong, SY, Kim, YR & Cho, NH 2015, 'Functional manipulation of dendritic cells by photoswitchable generation of intracellular reactive oxygen species', ACS Chemical Biology, vol. 10, no. 3, pp. 757-765. https://doi.org/10.1021/cb5009124

Functional manipulation of dendritic cells by photoswitchable generation of intracellular reactive oxygen species. / Cheong, Taek Chin; Shin, Eon Pil; Kwon, Eun Kyung; Choi, Ji Hye; Wang, Kang Kyun; Sharma, Prashant; Choi, Kyong Hoon; Lim, Jin Muk; Kim, Hong Gee; Oh, Keunhee; Jeon, Ju Hong; So, Insuk; Kim, In Gyu; Choi, Myung Sik; Kim, Young Keun; Seong, Seung Yong; Kim, Yong Rok; Cho, Nam Hyuk.

In: ACS Chemical Biology, Vol. 10, No. 3, 20.03.2015, p. 757-765.

Research output: Contribution to journalArticle

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T1 - Functional manipulation of dendritic cells by photoswitchable generation of intracellular reactive oxygen species

AU - Cheong, Taek Chin

AU - Shin, Eon Pil

AU - Kwon, Eun Kyung

AU - Choi, Ji Hye

AU - Wang, Kang Kyun

AU - Sharma, Prashant

AU - Choi, Kyong Hoon

AU - Lim, Jin Muk

AU - Kim, Hong Gee

AU - Oh, Keunhee

AU - Jeon, Ju Hong

AU - So, Insuk

AU - Kim, In Gyu

AU - Choi, Myung Sik

AU - Kim, Young Keun

AU - Seong, Seung Yong

AU - Kim, Yong Rok

AU - Cho, Nam Hyuk

PY - 2015/3/20

Y1 - 2015/3/20

N2 - Reactive oxygen species (ROS) play an important role in cellular signaling as second messengers. However, studying the role of ROS in physiological redox signaling has been hampered by technical difficulties in controlling their generation within cells. Here, we utilize two inert components, a photosensitizer and light, to finely manipulate the generation of intracellular ROS and examine their specific role in activating dendritic cells (DCs). Photoswitchable generation of intracellular ROS rapidly induced cytosolic mobilization of Ca2+, differential activation of mitogen-activated protein kinases, and nuclear translocation of NF-κB. Moreover, a transient intracellular ROS surge could activate immature DCs to mature and potently enhance migration in vitro and in vivo. Finally, we observed that intracellular ROS-stimulated DCs enhanced antigen specific T-cell responses in vitro and in vivo, which led to delayed tumor growth and prolonged survival of tumor-bearing mice when immunized with a specific tumor antigen. Therefore, a transient intracellular ROS surge alone, if properly manipulated, can cause immature DCs to differentiate into a motile state and mature forms that are sufficient to initiate adaptive T cell responses in vivo.

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