Functional polymorphism in H2BFWT-5′UTR is associated with susceptibility to male infertility

Jinu Lee, Hee Suk Park, Hwan Hee Kim, Yeo Jin Yun, Dong Ryul Lee, Suman Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

H2B histone family, member W, testis-specific (H2BFWT) gene encodes a testis-specific histone that becomes incorporated into sperm chromatin. A male infertility-associated single nucleotide polymorphism (-9C > T) within the 5′ untranslated region (5′UTR) of the H2BFWT gene was identified by direct sequencing. Statistical association studies showed the polymorphism significantly associated with male infertility (n = 442, P = 0.0157), especially in non-azoospermia (n = 262, P = 0.018). Furthermore, this polymorphism is also associated with sperm parameters, especially sperm count (n = 164, P = 0.0127) and vitality (n = 164, P = 0.0076). We investigated how the genetic variant at 5′UTR confers susceptibility to non-azoospermia. Western blotting of His-tag H2BFWT revealed a difference at the translational level between -9T and the wild-type -9C in the absence of change at the transcriptional level. Reporter assays showed that this reducing translational change originated from an upstream open reading frame (uORF) generated by the -9C to -9T change. Finally, in vivo H2BFWT expression in sperm was significantly dependent on the -9C > T genotype from non-azoospermia (P = 0.0061). Therefore, this polymorphism could affect the translational efficiency of a quantitatively important histone protein by the uORF. Our data implicate H2BFWT as a susceptibility factor for male infertility, possibly with other genetic and environmental factors.

Original languageEnglish
Pages (from-to)1942-1951
Number of pages10
JournalJournal of Cellular and Molecular Medicine
Volume13
Issue number8 B
DOIs
Publication statusPublished - 2009 Aug 1

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Cell Biology

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