Skeletal muscle has an inherent capacity for spontaneous regeneration. However, recovery after severe injuries such as volumetric muscle loss (VML) is limited. There is therefore a need to develop interventions to induce functional skeletal muscle restoration. One suggested approach includes tissue-engineered muscle constructs. Tissue-engineering treatments have so far been impeded by the lack of reliable cell sources and the challenges in engineering of suitable tissue scaffolds. To address these challenges, muscle extracellular matrix (MEM) and induced skeletal myogenic progenitor cells (iMPCs) are integrated within thermally drawn fiber based microchannel scaffolds. The microchannel fibers decorated with MEM enhance differentiation and maturation of iMPCs. Furthermore, engraftment of these bioengineered hybrid muscle constructs induce de novo muscle regeneration accompanied with microvessel and neuromuscular junction formation in a VML mouse model, ultimately leading to functional recovery of muscle activity.
Bibliographical noteFunding Information:
Y.J., D.S., E.J.J., and S.P. contributed equally to this work. This work was supported by LG Yonam Foundation of Korea (S.‐W.C.) and the Institute for Basic Science (IBS‐R026‐D1). The work was supported in part by Brain Korea 21 (BK21) FOUR program. P.A. acknowledges support from the National Institute for Neurological Disorders and Stroke (5R01NS086804), National Science Foundation (DMR‐1419807, EEC‐1028725), and the McGovern Institute for Brain Research at Massachusetts Institute of Technology. D.S. would like to thank the Craig Neilsen Spinal Cord Injury Foundation for a Post‐Doctoral Fellowship. Yoonhee Jin, Dena Shahriari, Eun Je Jeon, and Seongjun Park contributed equally to this work. The animal experimental procedures for the volumetric muscle loss (VML) model were approved by the Institutional Animal Care and Use Committee (IACUC) of Yonsei University (permit number: IACUC‐A‐201711‐657‐03) and Yonsei University Health System (permit number: 2019‐0270).
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Mechanics of Materials
- Mechanical Engineering