Functionalized heparin-protamine based self-assembled nanocomplex for efficient anti-angiogenic therapy

Farzana Alam, Taslim A. Al-Hilal, Seung Woo Chung, Jooho Park, Foyez Mahmud, Donghyun Seo, Han Sung Kim, Dong Soo Lee, Youngro Byun

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Angiogenesis is a key feature of cancer development, thus it is a good target for cancer therapy. However, drugs that have been designed to block angiogenesis mainly capture growth factors in circulation, resulting not only in the transient inhibition of tumor progression but also in producing undesirable side effects. Nanoparticular drug delivery systems, on the other hand, may help overcome such drawbacks and improve the efficacy of anti-angiogenic therapies by altering the biodistribution and pharmacokinetics, improving tumor targeting ability, and reducing side effects. In this light, we propose a new approach of anti-angiogenic therapy that combines strategies of long circulating, passive tumor targeting, and anti-angiogenesis efficacy using a new polyelectrolyte complex system that combines LHT7, a previously developed heparin-based angiogenesis inhibitor, with a protamine to form a self-assembling nanocomplex with a mean diameter of 200 nm, which is effective for anti-angiogenesis therapy. At first, LHT7 was modified with polyethylene glycol (PEG). We observed that PEG-LHT7/protamine nanocomplex was stable in buffer and slowly dissociated in plasma (9% dissociation for 24 h). Compared to the free form of PEG-LHT7, the mean residence time of PEG-LHT7/protamine nanocomplex was found higher (15.9 h) with its increased accumulation in tumor. Most importantly, PEG-LHT7/protamine nanocomplex was diffused and extravasated through the dense collagen matrix of the tumor. Thus, the study describes a successful application of functionalized PEG-LHT/protamine nanocomplex that can inhibit angiogenesis with long circulating, passive targeting, and tumor extravasating ability.

Original languageEnglish
Pages (from-to)180-189
Number of pages10
JournalJournal of Controlled Release
Volume197
DOIs
Publication statusPublished - 2015 Jan 10

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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