Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor

Seung Hyun Yang, Dan Heo, Eugene Lee, Eunjung Kim, Eun Kyung Lim, Young Han Lee, Seungjoo Haam, Jinsuck Suh, yongmin Huh, Jaemoon Yang, Sahng Wook Park

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by several methods in ASGPr-expressing HepG2 cells as target cells and ASGPr-deficient MCF7 cells. Physical and chemical properties of G-MFNP were examined using Fourier-transform infrared spectroscopy, dynamic light scattering, zeta potential analysis, and transmission electron microscopy. No significant cytotoxicity was observed in either cell line. Targeting ability was assessed using flow cytometry, magnetic resonance imaging, inductively coupled plasma atomic emission spectroscopy, absorbance analysis, dark-field microscopy, Prussian blue staining, and transmission electron microscopy. We demonstrated that G-MFNP target successfully and bind to ASGPr-expressing HepG2 cells specifically. We suggest that these results will be useful in strategies for cancer diagnoses based on magnetic resonance imaging.

Original languageEnglish
Article number475103
JournalNanotechnology
Volume24
Issue number47
DOIs
Publication statusPublished - 2013 Nov 29

Fingerprint

Asialoglycoprotein Receptor
Manganese
Ferrite
Biomarkers
Nanoparticles
Imaging techniques
Magnetic resonance
Bearings (structural)
Cells
Atomic emission spectroscopy
Transmission electron microscopy
Flow cytometry
Inductively coupled plasma
Dynamic light scattering
Zeta potential
Cell membranes
Cytotoxicity
Chemical properties
Fourier transform infrared spectroscopy
Microscopic examination

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Yang, S. H., Heo, D., Lee, E., Kim, E., Lim, E. K., Lee, Y. H., ... Park, S. W. (2013). Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor. Nanotechnology, 24(47), [475103]. https://doi.org/10.1088/0957-4484/24/47/475103
Yang, Seung Hyun ; Heo, Dan ; Lee, Eugene ; Kim, Eunjung ; Lim, Eun Kyung ; Lee, Young Han ; Haam, Seungjoo ; Suh, Jinsuck ; Huh, yongmin ; Yang, Jaemoon ; Park, Sahng Wook. / Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor. In: Nanotechnology. 2013 ; Vol. 24, No. 47.
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abstract = "Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by several methods in ASGPr-expressing HepG2 cells as target cells and ASGPr-deficient MCF7 cells. Physical and chemical properties of G-MFNP were examined using Fourier-transform infrared spectroscopy, dynamic light scattering, zeta potential analysis, and transmission electron microscopy. No significant cytotoxicity was observed in either cell line. Targeting ability was assessed using flow cytometry, magnetic resonance imaging, inductively coupled plasma atomic emission spectroscopy, absorbance analysis, dark-field microscopy, Prussian blue staining, and transmission electron microscopy. We demonstrated that G-MFNP target successfully and bind to ASGPr-expressing HepG2 cells specifically. We suggest that these results will be useful in strategies for cancer diagnoses based on magnetic resonance imaging.",
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Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor. / Yang, Seung Hyun; Heo, Dan; Lee, Eugene; Kim, Eunjung; Lim, Eun Kyung; Lee, Young Han; Haam, Seungjoo; Suh, Jinsuck; Huh, yongmin; Yang, Jaemoon; Park, Sahng Wook.

In: Nanotechnology, Vol. 24, No. 47, 475103, 29.11.2013.

Research output: Contribution to journalArticle

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T1 - Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor

AU - Yang, Seung Hyun

AU - Heo, Dan

AU - Lee, Eugene

AU - Kim, Eunjung

AU - Lim, Eun Kyung

AU - Lee, Young Han

AU - Haam, Seungjoo

AU - Suh, Jinsuck

AU - Huh, yongmin

AU - Yang, Jaemoon

AU - Park, Sahng Wook

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Y1 - 2013/11/29

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