Galectin-3 expression and BRAF mutation in cases of cytologically suspicious papillary thyroid carcinoma

Dokyung Kim, Hyunki Kim, Jinyoung Kwak, Minju Kim, Hyung Jae Jung, Ja Seung Koo, Beom Jin Lim, Chankwon Jung, Soon Won Hong

Research output: Contribution to journalArticle

Abstract

Background: Fine needle aspiration, which is known as the most accurate and cost-effective method for diagnosis of thyroid nodule, still may result in indeterminate cases that are pauci-cellular and show minor nuclear atypia, but most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF) mutation was found in about half of PTCs and galectin-3 was expressed by malignant tumors, helping us to differentiate malignancies from benign lesions. Methods: Cases studied included histologically 44 confirmed PTC cases and 18 benign cases previously diagnosed as suspicious of PTC using cytologic examination. Cases were analyzed for galectin-3 expression by immunohistochemical staining and BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme. Results: All 44 cases of PTC and 8 of 18 benign controls expressed galectin-3. BRAF mutations were found in only 9 of the 44 PTC cases. Assessment of galectin-3 expression demonstrated high sensitivity but low specificity. Evaluation of BRAF mutation revealed high specificity and low sensitivity. Conclusions: This study suggests that the combined application of these two methods for PTC of suspicious cytology is complementary.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalKorean Journal of Pathology
Volume44
Issue number2
DOIs
Publication statusPublished - 2010 Apr 1

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Proto-Oncogene Proteins B-raf
Galectin 3
Mutation
Sensitivity and Specificity
Thyroid Nodule
Factor IX
Fine Needle Biopsy
Restriction Fragment Length Polymorphisms
Cell Biology
Papillary Thyroid cancer
Neoplasms
Staining and Labeling
Costs and Cost Analysis
Polymerase Chain Reaction
Enzymes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Kim, Dokyung ; Kim, Hyunki ; Kwak, Jinyoung ; Kim, Minju ; Jung, Hyung Jae ; Koo, Ja Seung ; Lim, Beom Jin ; Jung, Chankwon ; Hong, Soon Won. / Galectin-3 expression and BRAF mutation in cases of cytologically suspicious papillary thyroid carcinoma. In: Korean Journal of Pathology. 2010 ; Vol. 44, No. 2. pp. 191-198.
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Galectin-3 expression and BRAF mutation in cases of cytologically suspicious papillary thyroid carcinoma. / Kim, Dokyung; Kim, Hyunki; Kwak, Jinyoung; Kim, Minju; Jung, Hyung Jae; Koo, Ja Seung; Lim, Beom Jin; Jung, Chankwon; Hong, Soon Won.

In: Korean Journal of Pathology, Vol. 44, No. 2, 01.04.2010, p. 191-198.

Research output: Contribution to journalArticle

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T1 - Galectin-3 expression and BRAF mutation in cases of cytologically suspicious papillary thyroid carcinoma

AU - Kim, Dokyung

AU - Kim, Hyunki

AU - Kwak, Jinyoung

AU - Kim, Minju

AU - Jung, Hyung Jae

AU - Koo, Ja Seung

AU - Lim, Beom Jin

AU - Jung, Chankwon

AU - Hong, Soon Won

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Y1 - 2010/4/1

N2 - Background: Fine needle aspiration, which is known as the most accurate and cost-effective method for diagnosis of thyroid nodule, still may result in indeterminate cases that are pauci-cellular and show minor nuclear atypia, but most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF) mutation was found in about half of PTCs and galectin-3 was expressed by malignant tumors, helping us to differentiate malignancies from benign lesions. Methods: Cases studied included histologically 44 confirmed PTC cases and 18 benign cases previously diagnosed as suspicious of PTC using cytologic examination. Cases were analyzed for galectin-3 expression by immunohistochemical staining and BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme. Results: All 44 cases of PTC and 8 of 18 benign controls expressed galectin-3. BRAF mutations were found in only 9 of the 44 PTC cases. Assessment of galectin-3 expression demonstrated high sensitivity but low specificity. Evaluation of BRAF mutation revealed high specificity and low sensitivity. Conclusions: This study suggests that the combined application of these two methods for PTC of suspicious cytology is complementary.

AB - Background: Fine needle aspiration, which is known as the most accurate and cost-effective method for diagnosis of thyroid nodule, still may result in indeterminate cases that are pauci-cellular and show minor nuclear atypia, but most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF) mutation was found in about half of PTCs and galectin-3 was expressed by malignant tumors, helping us to differentiate malignancies from benign lesions. Methods: Cases studied included histologically 44 confirmed PTC cases and 18 benign cases previously diagnosed as suspicious of PTC using cytologic examination. Cases were analyzed for galectin-3 expression by immunohistochemical staining and BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme. Results: All 44 cases of PTC and 8 of 18 benign controls expressed galectin-3. BRAF mutations were found in only 9 of the 44 PTC cases. Assessment of galectin-3 expression demonstrated high sensitivity but low specificity. Evaluation of BRAF mutation revealed high specificity and low sensitivity. Conclusions: This study suggests that the combined application of these two methods for PTC of suspicious cytology is complementary.

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