Gap junctions contribute to astrocytic resistance against zinc toxicity

Jinu Lee, Yeong Shin Yim, Suk Jin Ko, Dong Goo Kim, Chul Hoon Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Astrocytic gap junctions have been implicated in the regulation of cell viability. High amounts of extracellular zinc, which is released during ischemia, seizure, and brain trauma, can be cytotoxic to astrocytes. We tested whether gap junction coupling between astrocytes plays an important role in modulating zinc toxicity in hippocampal astrocytes. Zinc induces cell death in a dose-dependent manner in primary cultured hippocampal astrocytes. Two gap junction inhibitors, 18β-glycyrrhetinic acid and arachidonic acid, had no effect on zinc-induced cell death in low-confluence culture, where physical separation prevents gap junctions from forming. However, these inhibitors can potentiate zinc toxicity in high-confluence astrocyte cultures. Zinc toxicity was substantially suppressed upon connexin 43 overexpression, whereas knockdown caused a significant enhancement of the toxicity in high-confluence cultures. These data suggest that gap junctions in hippocampal astrocytes provide a protective role against zinc toxicity.

Original languageEnglish
Pages (from-to)314-318
Number of pages5
JournalBrain Research Bulletin
Volume86
Issue number5-6
DOIs
Publication statusPublished - 2011 Nov 25

Fingerprint

Gap Junctions
Zinc
Astrocytes
Cell Death
Glycyrrhetinic Acid
Connexin 43
Arachidonic Acid
Cell Survival
Seizures
Ischemia

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Lee, Jinu ; Yim, Yeong Shin ; Ko, Suk Jin ; Kim, Dong Goo ; Kim, Chul Hoon. / Gap junctions contribute to astrocytic resistance against zinc toxicity. In: Brain Research Bulletin. 2011 ; Vol. 86, No. 5-6. pp. 314-318.
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Gap junctions contribute to astrocytic resistance against zinc toxicity. / Lee, Jinu; Yim, Yeong Shin; Ko, Suk Jin; Kim, Dong Goo; Kim, Chul Hoon.

In: Brain Research Bulletin, Vol. 86, No. 5-6, 25.11.2011, p. 314-318.

Research output: Contribution to journalArticle

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