Gastric tumor development in Smad3-deficient mice initiates from forestomach/glandular transition zone along the lesser curvature

KiTaek Nam, Ryan O'Neal, Yeo Song Lee, Yongchan Lee, Robert J. Coffey, James R. Goldenring

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

SMAD proteins are downstream effectors of the TGF-Β signaling pathway. Smad3-null mice develop colorectal cancer by 6 months of age. In this study, we have examined whether the loss of Smad3 promotes gastric neoplasia in mice. The stomachs of Smad3 -/-mice were compared with age-matched Smad3 heterozygous and wild-type mice. E-cadherin, Ki-67, phosphoSTAT3, and TFF2/SP expression was analyzed by immunohistochemisty. The short hairpin RNA (ShRNA)-mediated knockdown of Smad3 in AGS and MKN28 cells was also performed. In addition, we examined alterations in DCLK1-expressing cells. Smad3/mouse stomachs at 6 months of age revealed the presence of exophytic growths along the lesser curvature in the proximal fundus. Six-month-old Smad3/mouse stomachs showed metaplastic columnar glands initiating from the transition zone junction between the forestomach and the glandular epithelium along the lesser curvature. Ten-month-old Smad3 -/-mice all exhibited invasive gastric neoplastic changes with increased Ki-67, phosphoSTAT3 expression, and aberrant cytosolic E-cadherin staining in papillary glands within the invading submucosal gland. The shRNA-mediated knockdown of Smad3 in AGS and MKN28 cells promoted the expression of phosphoSTAT3. DCLK1-expressing cells, which also stained for the tuft cell marker acetylated-α-tubulin, were observed in 10-month-old Smad3 -/-mice within tumors and in fundic invasive lesions. In conclusion, Smad3-null mice develop gastric tumors in the fundus, which arise from the junction between the forestomach and the glandular epithelium and progress to prominent invasive tumors over time. Smad3-null mice represent a novel model of fundic gastric tumor initiated from forestomach/glandular transition zone along the lesser curvature.

Original languageEnglish
Pages (from-to)883-895
Number of pages13
JournalLaboratory Investigation
Volume92
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1

Fingerprint

Stomach
Neoplasms
Cadherins
Small Interfering RNA
Epithelium
Tubulin
Colorectal Neoplasms
Staining and Labeling
Growth

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

Nam, KiTaek ; O'Neal, Ryan ; Lee, Yeo Song ; Lee, Yongchan ; Coffey, Robert J. ; Goldenring, James R. / Gastric tumor development in Smad3-deficient mice initiates from forestomach/glandular transition zone along the lesser curvature. In: Laboratory Investigation. 2012 ; Vol. 92, No. 6. pp. 883-895.
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abstract = "SMAD proteins are downstream effectors of the TGF-Β signaling pathway. Smad3-null mice develop colorectal cancer by 6 months of age. In this study, we have examined whether the loss of Smad3 promotes gastric neoplasia in mice. The stomachs of Smad3 -/-mice were compared with age-matched Smad3 heterozygous and wild-type mice. E-cadherin, Ki-67, phosphoSTAT3, and TFF2/SP expression was analyzed by immunohistochemisty. The short hairpin RNA (ShRNA)-mediated knockdown of Smad3 in AGS and MKN28 cells was also performed. In addition, we examined alterations in DCLK1-expressing cells. Smad3/mouse stomachs at 6 months of age revealed the presence of exophytic growths along the lesser curvature in the proximal fundus. Six-month-old Smad3/mouse stomachs showed metaplastic columnar glands initiating from the transition zone junction between the forestomach and the glandular epithelium along the lesser curvature. Ten-month-old Smad3 -/-mice all exhibited invasive gastric neoplastic changes with increased Ki-67, phosphoSTAT3 expression, and aberrant cytosolic E-cadherin staining in papillary glands within the invading submucosal gland. The shRNA-mediated knockdown of Smad3 in AGS and MKN28 cells promoted the expression of phosphoSTAT3. DCLK1-expressing cells, which also stained for the tuft cell marker acetylated-α-tubulin, were observed in 10-month-old Smad3 -/-mice within tumors and in fundic invasive lesions. In conclusion, Smad3-null mice develop gastric tumors in the fundus, which arise from the junction between the forestomach and the glandular epithelium and progress to prominent invasive tumors over time. Smad3-null mice represent a novel model of fundic gastric tumor initiated from forestomach/glandular transition zone along the lesser curvature.",
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Gastric tumor development in Smad3-deficient mice initiates from forestomach/glandular transition zone along the lesser curvature. / Nam, KiTaek; O'Neal, Ryan; Lee, Yeo Song; Lee, Yongchan; Coffey, Robert J.; Goldenring, James R.

In: Laboratory Investigation, Vol. 92, No. 6, 01.06.2012, p. 883-895.

Research output: Contribution to journalArticle

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