Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor

Dong Joon Lee, Chengri Jin, Eun Jung Kim, Jong Min Lee, Han Sung Jung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide’s receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalHistochemistry and cell biology
Volume144
Issue number3
DOIs
Publication statusPublished - 2015 Sep 17

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All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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