Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor

Dong Joon Lee, Chengri Jin, Eun Jung Kim, Jong Min Lee, Han Sung Jung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide’s receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalHistochemistry and cell biology
Volume144
Issue number3
DOIs
Publication statusPublished - 2015 Sep 17

Fingerprint

Gastrin-Releasing Peptide
Incisor
Ameloblasts
Dentin
Dental Enamel
Tooth Calcification
Calcium
Odontoblasts
Peptide Receptors
G-Protein-Coupled Receptors
Kidney Transplantation
Capsules
In Situ Hybridization
Intercellular Signaling Peptides and Proteins
Epithelium

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Lee, Dong Joon ; Jin, Chengri ; Kim, Eun Jung ; Lee, Jong Min ; Jung, Han Sung. / Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor. In: Histochemistry and cell biology. 2015 ; Vol. 144, No. 3. pp. 273-279.
@article{ed76beab645140b386e8b77eb671cff1,
title = "Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor",
abstract = "Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide’s receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.",
author = "Lee, {Dong Joon} and Chengri Jin and Kim, {Eun Jung} and Lee, {Jong Min} and Jung, {Han Sung}",
year = "2015",
month = "9",
day = "17",
doi = "10.1007/s00418-015-1335-1",
language = "English",
volume = "144",
pages = "273--279",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "3",

}

Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor. / Lee, Dong Joon; Jin, Chengri; Kim, Eun Jung; Lee, Jong Min; Jung, Han Sung.

In: Histochemistry and cell biology, Vol. 144, No. 3, 17.09.2015, p. 273-279.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor

AU - Lee, Dong Joon

AU - Jin, Chengri

AU - Kim, Eun Jung

AU - Lee, Jong Min

AU - Jung, Han Sung

PY - 2015/9/17

Y1 - 2015/9/17

N2 - Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide’s receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.

AB - Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide’s receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.

UR - http://www.scopus.com/inward/record.url?scp=84939254470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939254470&partnerID=8YFLogxK

U2 - 10.1007/s00418-015-1335-1

DO - 10.1007/s00418-015-1335-1

M3 - Article

C2 - 26126650

AN - SCOPUS:84939254470

VL - 144

SP - 273

EP - 279

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 3

ER -