GC-MS Analysis of Various Phytoestrogens in Health Functional Foods

Yu Ra Kim, Hee Soo Pyo, Bong Chul Chung, Myeong Hee Moon, Jeongae Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography–mass spectrometry (GC-MS) for the monitoring of 21 phytoestrogens was developed and fully validated. Phytoestrogens were extracted by liquid–liquid extraction using methyl tert-butylether as the solvent. Food samples were enzymatically hydrolyzed with β-glucuronidase/arylsulfatase. Before GC-MS, the analytes were derivatized to their trimethylsilyl derivatives by incubation with N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for 30 min at 80°C. The calibration curves showed good linearity (r 2 > 0.99) in the range of interest for each analyte. The limits of detection and quantification of the 21 phytoestrogens were in the ranges of 0.5–20 and 1–50 ng/mL, respectively. The intra- and inter-day precisions were all below 15.7%. These multivariate procedures were efficient in determining the optimal conditions using peak heights as responses.

Original languageEnglish
Pages (from-to)448-458
Number of pages11
JournalBulletin of the Korean Chemical Society
Volume38
Issue number4
DOIs
Publication statusPublished - 2017 Apr 1

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Phytoestrogens
Spectrometry
Gases
Health
Arylsulfatases
Glucuronidase
Calibration
Derivatives
Functional food
Monitoring

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Kim, Yu Ra ; Pyo, Hee Soo ; Chung, Bong Chul ; Moon, Myeong Hee ; Lee, Jeongae. / GC-MS Analysis of Various Phytoestrogens in Health Functional Foods. In: Bulletin of the Korean Chemical Society. 2017 ; Vol. 38, No. 4. pp. 448-458.
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GC-MS Analysis of Various Phytoestrogens in Health Functional Foods. / Kim, Yu Ra; Pyo, Hee Soo; Chung, Bong Chul; Moon, Myeong Hee; Lee, Jeongae.

In: Bulletin of the Korean Chemical Society, Vol. 38, No. 4, 01.04.2017, p. 448-458.

Research output: Contribution to journalArticle

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