Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors

F. Yang; J. J. Green; T. Dinio; L. Keung; S. W. Cho; H. Park; R. Langer; D. G. Anderson

doi:10.1038/gt.2008.182

Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors

F. Yang, J. J. Green, T. Dinio, L. Keung, S. W. Cho, H. Park, R. Langer, D. G. Anderson

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

Gene delivery to stem cells holds great potential for tissue regeneration and delivery of therapeutic proteins. The major barrier is the lack of safe and efficient delivery methods. Here, we report enhanced gene delivery systems for human stem cells using biodegradable polymeric vectors. A library of poly (β-amino esters) end-modified derivatives was developed and optimized for high transfection efficiency and low cytotoxicity for three human stem cell lines including human mesenchymal stem cells (hMSCs), human adipose-derived stem cells (hADSCs) and human embryonic stem cell-derived cells (hESCds). In the presence of 10% serum, leading end-modified C32 polymeric vectors exhibited significantly high transfection efficiency in hMSCs (27±2%), hADSCs (24±3%) and hESCds (56±11%), with high cell viability (87-97%) achieved in all cell types. Our results show that poly(β-amino esters) as a class, and end-modified versions of C32 in particular, are efficient polymeric vectors for gene delivery to both adult and embryonic-derived stem cells.

Original language	English
Pages (from-to)	533-546
Number of pages	14
Journal	Gene Therapy
Volume	16
Issue number	4
DOIs	https://doi.org/10.1038/gt.2008.182
Publication status	Published - 2009

Bibliographical note

Funding Information:
We thank the NIH (R01-EB000244-27 and R01-DE016516-03) for funding. FY gratefully acknowledge the National Institutes of Health for National Research Service Award postdoctoral fellowship (1F32 AR056567-01). We also thank the laboratory of Professor Johnny Huard at the University of Pittsburgh for kindly providing us the DNA plasmid-encoding human vascular endothelial growth factor 165 used in this study.

All Science Journal Classification (ASJC) codes

Molecular Medicine
Molecular Biology
Genetics

Access to Document

10.1038/gt.2008.182

Cite this

@article{acc47248e6184165be753d0c06f727a1,

title = "Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors",

abstract = "Gene delivery to stem cells holds great potential for tissue regeneration and delivery of therapeutic proteins. The major barrier is the lack of safe and efficient delivery methods. Here, we report enhanced gene delivery systems for human stem cells using biodegradable polymeric vectors. A library of poly (β-amino esters) end-modified derivatives was developed and optimized for high transfection efficiency and low cytotoxicity for three human stem cell lines including human mesenchymal stem cells (hMSCs), human adipose-derived stem cells (hADSCs) and human embryonic stem cell-derived cells (hESCds). In the presence of 10% serum, leading end-modified C32 polymeric vectors exhibited significantly high transfection efficiency in hMSCs (27±2%), hADSCs (24±3%) and hESCds (56±11%), with high cell viability (87-97%) achieved in all cell types. Our results show that poly(β-amino esters) as a class, and end-modified versions of C32 in particular, are efficient polymeric vectors for gene delivery to both adult and embryonic-derived stem cells.",

author = "F. Yang and Green, {J. J.} and T. Dinio and L. Keung and Cho, {S. W.} and H. Park and R. Langer and Anderson, {D. G.}",

note = "Funding Information: We thank the NIH (R01-EB000244-27 and R01-DE016516-03) for funding. FY gratefully acknowledge the National Institutes of Health for National Research Service Award postdoctoral fellowship (1F32 AR056567-01). We also thank the laboratory of Professor Johnny Huard at the University of Pittsburgh for kindly providing us the DNA plasmid-encoding human vascular endothelial growth factor 165 used in this study.",

year = "2009",

doi = "10.1038/gt.2008.182",

language = "English",

volume = "16",

pages = "533--546",

journal = "Gene Therapy",

issn = "0969-7128",

publisher = "Nature Publishing Group",

number = "4",

}

TY - JOUR

T1 - Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors

AU - Yang, F.

AU - Green, J. J.

AU - Dinio, T.

AU - Keung, L.

AU - Cho, S. W.

AU - Park, H.

AU - Langer, R.

AU - Anderson, D. G.

N1 - Funding Information: We thank the NIH (R01-EB000244-27 and R01-DE016516-03) for funding. FY gratefully acknowledge the National Institutes of Health for National Research Service Award postdoctoral fellowship (1F32 AR056567-01). We also thank the laboratory of Professor Johnny Huard at the University of Pittsburgh for kindly providing us the DNA plasmid-encoding human vascular endothelial growth factor 165 used in this study.

PY - 2009

Y1 - 2009

N2 - Gene delivery to stem cells holds great potential for tissue regeneration and delivery of therapeutic proteins. The major barrier is the lack of safe and efficient delivery methods. Here, we report enhanced gene delivery systems for human stem cells using biodegradable polymeric vectors. A library of poly (β-amino esters) end-modified derivatives was developed and optimized for high transfection efficiency and low cytotoxicity for three human stem cell lines including human mesenchymal stem cells (hMSCs), human adipose-derived stem cells (hADSCs) and human embryonic stem cell-derived cells (hESCds). In the presence of 10% serum, leading end-modified C32 polymeric vectors exhibited significantly high transfection efficiency in hMSCs (27±2%), hADSCs (24±3%) and hESCds (56±11%), with high cell viability (87-97%) achieved in all cell types. Our results show that poly(β-amino esters) as a class, and end-modified versions of C32 in particular, are efficient polymeric vectors for gene delivery to both adult and embryonic-derived stem cells.

AB - Gene delivery to stem cells holds great potential for tissue regeneration and delivery of therapeutic proteins. The major barrier is the lack of safe and efficient delivery methods. Here, we report enhanced gene delivery systems for human stem cells using biodegradable polymeric vectors. A library of poly (β-amino esters) end-modified derivatives was developed and optimized for high transfection efficiency and low cytotoxicity for three human stem cell lines including human mesenchymal stem cells (hMSCs), human adipose-derived stem cells (hADSCs) and human embryonic stem cell-derived cells (hESCds). In the presence of 10% serum, leading end-modified C32 polymeric vectors exhibited significantly high transfection efficiency in hMSCs (27±2%), hADSCs (24±3%) and hESCds (56±11%), with high cell viability (87-97%) achieved in all cell types. Our results show that poly(β-amino esters) as a class, and end-modified versions of C32 in particular, are efficient polymeric vectors for gene delivery to both adult and embryonic-derived stem cells.

UR - http://www.scopus.com/inward/record.url?scp=64549140262&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64549140262&partnerID=8YFLogxK

U2 - 10.1038/gt.2008.182

DO - 10.1038/gt.2008.182

M3 - Article

C2 - 19129861

AN - SCOPUS:64549140262

SN - 0969-7128

VL - 16

SP - 533

EP - 546

JO - Gene Therapy

JF - Gene Therapy

IS - 4

ER -

Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this