Gene expression patterns in glucose-stimulated podocytes

Seung Hyeok Han, Sanghwa Yang, Dong Sub Jung, Jin Ji Li, Jin Ju Kim, Seung Jae Kwak, Dong Ki Kim, Sung Jin Moon, Jung Eun Lee, Dae Suk Han, Shin Wook Kang

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Abstract

To explore the mechanisms of podocyte injury under diabetic conditions, we performed an expression profile in glucose-stimulated podocytes. Differential gene expression profiles between conditionally immortalized mouse podocytes cultured in medium containing 5.6 and 30 mM glucose were measured with oligonucleotide microarrays. Of the genes identified, heme oxygenase-1, vascular endothelial growth factor-A, and thrombospondin-1 showed a consistently increased pattern, whereas angiotensin-converting enzyme-2 and peroxisomal proliferator activator receptor-γ were down-regulated. These results were validated using real-time PCR and western blotting in podocytes, and with immunohistochemistry on renal tissues from streptozotocin-induced diabetic rats. Not only is this the first report of gene expression profiling of podocyte injury under diabetic conditions, but the identified genes are promising targets for future diabetes research.

Original languageEnglish
Pages (from-to)514-518
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume370
Issue number3
DOIs
Publication statusPublished - 2008 Jun 6

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Han, S. H., Yang, S., Jung, D. S., Li, J. J., Kim, J. J., Kwak, S. J., Kim, D. K., Moon, S. J., Lee, J. E., Han, D. S., & Kang, S. W. (2008). Gene expression patterns in glucose-stimulated podocytes. Biochemical and Biophysical Research Communications, 370(3), 514-518. https://doi.org/10.1016/j.bbrc.2008.03.121