Gene expression profiling of KBH-A42, a novel histone deacetylase inhibitor, in human leukemia and bladder cancer cell lines

Moo Rim Kang, Jong Soon Kang, Jeong Wook Yang, Bo Geun Kim, Jin Ah Kim, Yeong Nang Jo, Kiho Lee, Chang Woo Lee, Ki Hoon Lee, Jieun Yun, Hwan Mook Kim, Gyoonhee Han, Jong Seong Kang, Song Kyu Park

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Abstract

The aim of this study was to investigate the anti-tumor activity of KBH-A42, a novel synthetic histone deacetylase (HDAC) inhibitor. KBH-A42 was shown to significantly suppress the proliferation of all 14 human cancer cell lines tested. Among these cell lines, the human leukemia cell line K562 was the most sensitive, whereas the UM-UC-3 bladder cancer cells were the least sensitive. Additionally, in a human tumor xenograft model using Balb/c nude mice, KBH-A42 was shown to significantly inhibit the growth of K562 tumors, although it only slightly inhibited the growth of UM-UC-3 tumors. The results of flow cytometry analysis and caspase 3/7 activation assays showed that the growth inhibition of K562 cells by KBH-A42 was mediated, at least in part, by the induction of apoptosis, but its growth inhibitory effects on UM-UC-3 cells were not mediated by apoptotic induction. In an effort to gain insight into the mechanism by which KBH-A42 inhibits the growth of cancer cells, a microarray analysis was conducted. Four genes were selected from the genes that were down-regulated or up-regulated by KBH-A42 and confirmed via reverse transcription-polymerase chain reaction as follows: Harakiri (HRK), tumor necrosis factor receptor superfamily, member 10b (TNFRSF10B), PYD and CARD domain containing protein gene (PYCARD) and tumor necrosis factor receptor superfamily, member 8 (TNFRSF8). Collectively, the in vitro and in vivo results suggested that KBH-A42 exhibits anti-cancer activity, but various types of cells may be regulated differentially by KBH-A42.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalOncology Letters
Volume3
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kang, M. R., Kang, J. S., Yang, J. W., Kim, B. G., Kim, J. A., Jo, Y. N., Lee, K., Lee, C. W., Lee, K. H., Yun, J., Kim, H. M., Han, G., Kang, J. S., & Park, S. K. (2012). Gene expression profiling of KBH-A42, a novel histone deacetylase inhibitor, in human leukemia and bladder cancer cell lines. Oncology Letters, 3(1), 113-118. https://doi.org/10.3892/ol.2011.430