Gene regulation by MAPK substrate competition

Yoosik Kim, María José Andreu, Bomyi Lim, Kwanghun Chung, Mark Terayama, Gerardo Jiménez, Celeste A. Berg, Hang Lu, Stanislav Y. Shvartsman

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Developing tissues are patterned by coordinated activities of signaling systems, which can be integrated by a regulatory region of a gene that binds multiple transcription factors or by a transcription factor that is modified by multiple enzymes. Based on a combination of genetic and imaging experiments in the early Drosophila embryo, we describe a signal integration mechanism that cannot be reduced to a single gene regulatory element or a single transcription factor. This mechanism relies on an enzymatic network formed by mitogen-activated protein kinase (MAPK) and its substrates. Specifically, anteriorly localized MAPK substrates, such as Bicoid, antagonize MAPK-dependent downregulation of Capicua, a repressor that is involved in gene regulation along the dorsoventral axis of the embryo. MAPK substrate competition provides a basis for ternary interaction of the anterior, dorsoventral, and terminal patterning systems. A mathematical model of this interaction can explain gene expression patterns with both anteroposterior and dorsoventral polarities.

Original languageEnglish
Pages (from-to)880-887
Number of pages8
JournalDevelopmental Cell
Volume20
Issue number6
DOIs
Publication statusPublished - 2011 Jun 14

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Mitogen-Activated Protein Kinases
Gene expression
Transcription Factors
Substrates
Genes
Embryonic Structures
Nucleic Acid Regulatory Sequences
Regulator Genes
Drosophila
Theoretical Models
Down-Regulation
Tissue
Mathematical models
Gene Expression
Imaging techniques
Enzymes
Experiments

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Cite this

Kim, Y., Andreu, M. J., Lim, B., Chung, K., Terayama, M., Jiménez, G., ... Shvartsman, S. Y. (2011). Gene regulation by MAPK substrate competition. Developmental Cell, 20(6), 880-887. https://doi.org/10.1016/j.devcel.2011.05.009
Kim, Yoosik ; Andreu, María José ; Lim, Bomyi ; Chung, Kwanghun ; Terayama, Mark ; Jiménez, Gerardo ; Berg, Celeste A. ; Lu, Hang ; Shvartsman, Stanislav Y. / Gene regulation by MAPK substrate competition. In: Developmental Cell. 2011 ; Vol. 20, No. 6. pp. 880-887.
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Kim, Y, Andreu, MJ, Lim, B, Chung, K, Terayama, M, Jiménez, G, Berg, CA, Lu, H & Shvartsman, SY 2011, 'Gene regulation by MAPK substrate competition', Developmental Cell, vol. 20, no. 6, pp. 880-887. https://doi.org/10.1016/j.devcel.2011.05.009

Gene regulation by MAPK substrate competition. / Kim, Yoosik; Andreu, María José; Lim, Bomyi; Chung, Kwanghun; Terayama, Mark; Jiménez, Gerardo; Berg, Celeste A.; Lu, Hang; Shvartsman, Stanislav Y.

In: Developmental Cell, Vol. 20, No. 6, 14.06.2011, p. 880-887.

Research output: Contribution to journalArticle

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Kim Y, Andreu MJ, Lim B, Chung K, Terayama M, Jiménez G et al. Gene regulation by MAPK substrate competition. Developmental Cell. 2011 Jun 14;20(6):880-887. https://doi.org/10.1016/j.devcel.2011.05.009