Abstract
Developing tissues are patterned by coordinated activities of signaling systems, which can be integrated by a regulatory region of a gene that binds multiple transcription factors or by a transcription factor that is modified by multiple enzymes. Based on a combination of genetic and imaging experiments in the early Drosophila embryo, we describe a signal integration mechanism that cannot be reduced to a single gene regulatory element or a single transcription factor. This mechanism relies on an enzymatic network formed by mitogen-activated protein kinase (MAPK) and its substrates. Specifically, anteriorly localized MAPK substrates, such as Bicoid, antagonize MAPK-dependent downregulation of Capicua, a repressor that is involved in gene regulation along the dorsoventral axis of the embryo. MAPK substrate competition provides a basis for ternary interaction of the anterior, dorsoventral, and terminal patterning systems. A mathematical model of this interaction can explain gene expression patterns with both anteroposterior and dorsoventral polarities.
| Original language | English |
|---|---|
| Pages (from-to) | 880-887 |
| Number of pages | 8 |
| Journal | Developmental Cell |
| Volume | 20 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2011 Jun 14 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Developmental Biology
- Cell Biology
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Gene regulation by MAPK substrate competition. / Kim, Yoosik; Andreu, María José; Lim, Bomyi; Chung, Kwanghun; Terayama, Mark; Jiménez, Gerardo; Berg, Celeste A.; Lu, Hang; Shvartsman, Stanislav Y.
In: Developmental Cell, Vol. 20, No. 6, 14.06.2011, p. 880-887.Research output: Contribution to journal › Article
TY - JOUR
T1 - Gene regulation by MAPK substrate competition
AU - Kim, Yoosik
AU - Andreu, María José
AU - Lim, Bomyi
AU - Chung, Kwanghun
AU - Terayama, Mark
AU - Jiménez, Gerardo
AU - Berg, Celeste A.
AU - Lu, Hang
AU - Shvartsman, Stanislav Y.
PY - 2011/6/14
Y1 - 2011/6/14
N2 - Developing tissues are patterned by coordinated activities of signaling systems, which can be integrated by a regulatory region of a gene that binds multiple transcription factors or by a transcription factor that is modified by multiple enzymes. Based on a combination of genetic and imaging experiments in the early Drosophila embryo, we describe a signal integration mechanism that cannot be reduced to a single gene regulatory element or a single transcription factor. This mechanism relies on an enzymatic network formed by mitogen-activated protein kinase (MAPK) and its substrates. Specifically, anteriorly localized MAPK substrates, such as Bicoid, antagonize MAPK-dependent downregulation of Capicua, a repressor that is involved in gene regulation along the dorsoventral axis of the embryo. MAPK substrate competition provides a basis for ternary interaction of the anterior, dorsoventral, and terminal patterning systems. A mathematical model of this interaction can explain gene expression patterns with both anteroposterior and dorsoventral polarities.
AB - Developing tissues are patterned by coordinated activities of signaling systems, which can be integrated by a regulatory region of a gene that binds multiple transcription factors or by a transcription factor that is modified by multiple enzymes. Based on a combination of genetic and imaging experiments in the early Drosophila embryo, we describe a signal integration mechanism that cannot be reduced to a single gene regulatory element or a single transcription factor. This mechanism relies on an enzymatic network formed by mitogen-activated protein kinase (MAPK) and its substrates. Specifically, anteriorly localized MAPK substrates, such as Bicoid, antagonize MAPK-dependent downregulation of Capicua, a repressor that is involved in gene regulation along the dorsoventral axis of the embryo. MAPK substrate competition provides a basis for ternary interaction of the anterior, dorsoventral, and terminal patterning systems. A mathematical model of this interaction can explain gene expression patterns with both anteroposterior and dorsoventral polarities.
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UR - http://www.scopus.com/inward/citedby.url?scp=79958195843&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2011.05.009
DO - 10.1016/j.devcel.2011.05.009
M3 - Article
C2 - 21664584
AN - SCOPUS:79958195843
VL - 20
SP - 880
EP - 887
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 6
ER -