Gene synthesis by circular assembly amplification

Duhee Bang, George M. Church

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Here we report the development of a gene-synthesis technology, circular assembly amplification. In this approach, we first constructed exonuclease-resistant circular DNA via simultaneous ligation of oligonucleotides. Exonuclease- and subsequent mismatch cleaving endonuclease-mediated degradation of the resulting ligation mixture eliminated error-rich products, thereby substantially improving gene-synthesis quality. We used this method to construct genes encoding a small thermostable DNA polymerase, a highly repetitive DNA sequence and large (>4 kb) constructs.

Original languageEnglish
Pages (from-to)37-39
Number of pages3
JournalNature Methods
Volume5
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1

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Exonucleases
Amplification
Genes
Circular DNA
Gene encoding
Ligation
Endonucleases
DNA sequences
DNA-Directed DNA Polymerase
Oligonucleotides
Nucleic Acid Repetitive Sequences
Degradation
Technology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Bang, Duhee ; Church, George M. / Gene synthesis by circular assembly amplification. In: Nature Methods. 2008 ; Vol. 5, No. 1. pp. 37-39.
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Gene synthesis by circular assembly amplification. / Bang, Duhee; Church, George M.

In: Nature Methods, Vol. 5, No. 1, 01.01.2008, p. 37-39.

Research output: Contribution to journalArticle

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