Gene-transferring efficiencies of novel diamino cationic lipids with varied hydrocarbon chains

Hong Sung Kim, Jaeho Moon, Keun Sik Kim, Myung Min Choi, Ji Eun Lee, Yeon Heo, Dae Hyan Cho, Doo Ok Jang, Yong Serk Park

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Utilizing three biocompatible components, a series of novel cationic lipids has been chemically synthesized and tested for their gene-transferring capabilities in 293 transformed kidney cells and B16BL6 mouse melanoma cells. The synthesized cationic lipids consisting of a core of lysine and aspartic acid with hydrocarbon chains of varied length were assigned the acronyms DLKD (O,O′-dilauryl N-lysylaspartate), DMKD (O,O′-dimyristyl N-lysylaspartate), DPKD (O,O′-dipalmityl N-lysylaspartate), and DSKD (O,O′-distearyl N-lysylaspartate). The gene-transferring capabilities of these cationic lipids were found to be dependent on the hydrocarbon chain length. Under similar experimental conditions, the order of gene transfection efficiency was DMKD > DLKD > DPKD > DSKD. Addition of cholesterol or dioleoyl phosphatidylethanolamine (DOPE) as a colipid did not change this order. Colipid addition affected the transfection efficiency positively or negatively depending on the length of the cationic lipid acyl chain. On the whole, the length of the hydrophobic carbon chain was a major factor governing the gene-transferring capabilities of this series of cationic lipids. The observed differences in transfection efficiency may be due to differing binding affinities to DNA molecules as well as differences in the surface charge potential of the liposome-DNA complexes (lipoplexes) in the aqueous environment.

Original languageEnglish
Pages (from-to)1095-1101
Number of pages7
JournalBioconjugate Chemistry
Volume15
Issue number5
DOIs
Publication statusPublished - 2004 Sep 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Kim, H. S., Moon, J., Kim, K. S., Choi, M. M., Lee, J. E., Heo, Y., Cho, D. H., Jang, D. O., & Park, Y. S. (2004). Gene-transferring efficiencies of novel diamino cationic lipids with varied hydrocarbon chains. Bioconjugate Chemistry, 15(5), 1095-1101. https://doi.org/10.1021/bc049934t