General and Facile Coating of Single Cells via Mild Reduction

Hyunbum Kim, Kwangsoo Shin, Ok Kyu Park, Daheui Choi, Hwan D. Kim, Seungmin Baik, Soo Hong Lee, Seung Hae Kwon, Kevin J. Yarema, Jinkee Hong, Taeghwan Hyeon, Nathaniel S. Hwang

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Abstract

Cell surface modification has been extensively studied to enhance the efficacy of cell therapy. Still, general accessibility and versatility are remaining challenges to meet the increasing demand for cell-based therapy. Herein, we present a facile and universal cell surface modification method that involves mild reduction of disulfide bonds in cell membrane protein to thiol groups. The reduced cells are successfully coated with biomolecules, polymers, and nanoparticles for an assortment of applications, including rapid cell assembly, in vivo cell monitoring, and localized cell-based drug delivery. No adverse effect on cellular morphology, viability, proliferation, and metabolism is observed. Furthermore, simultaneous coating with polyethylene glycol and dexamethasone-loaded nanoparticles facilitates enhanced cellular activities in mice, overcoming immune rejection.

Original languageEnglish
Pages (from-to)1199-1202
Number of pages4
JournalJournal of the American Chemical Society
Volume140
Issue number4
DOIs
Publication statusPublished - 2018 Jan 31

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All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Kim, H., Shin, K., Park, O. K., Choi, D., Kim, H. D., Baik, S., Lee, S. H., Kwon, S. H., Yarema, K. J., Hong, J., Hyeon, T., & Hwang, N. S. (2018). General and Facile Coating of Single Cells via Mild Reduction. Journal of the American Chemical Society, 140(4), 1199-1202. https://doi.org/10.1021/jacs.7b08440