Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes

Sun Ho Lee; Jaewon Oh; Seung Tae Lee; Dongju Won; Sangwoo Kim; Hyo Kyoung Choi; Seok Jun Kim; Hyunho Han; Minjae Yoon; Jong Rak Choi; Ho Geun Yoon; Sahng Wook Park; Seok Min Kang; Seung Hyun Lee

doi:10.1016/j.scr.2021.102629

Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes

Sun Ho Lee, Jaewon Oh, Seung Tae Lee, Dongju Won, Sangwoo Kim, Hyo Kyoung Choi, Seok Jun Kim, Hyunho Han, Minjae Yoon, Jong Rak Choi, Ho Geun Yoon, Sahng Wook Park, Seok Min Kang, Seung Hyun Lee

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Dilated cardiomyopathy (DCM) is a heart muscle disease that causes heart failure and is the leading cause for heart transplantation. It is a heart muscle disease resulted from a variety of genetics, toxic, metabolic, and infectious causes. One of the most prevalent genetic causes of DCM is a protein-truncating variant in the Titin gene (TTNtv). We have generated a human-induced pluripotent stem cell (hiPSC) line from patients who underwent heart transplantation due to DCM carrying a TTNtv mutation (c.70051C > T, p.Arg23351Ter) at the age of 20. The generated hiPSCs showed normal karyotype (46, XY) and expression of pluripotency markers, and were differentiated towards cardiomyocytes successfully.

Original language	English
Article number	102629
Journal	Stem Cell Research
Volume	59
DOIs	https://doi.org/10.1016/j.scr.2021.102629
Publication status	Published - 2022 Mar

Bibliographical note

Funding Information:
This work has supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2021R1I1A1A01060135, 2019R1C1C1002334, 2018R1D1A1B07049956). This study also was supported by Faculty research grant of Yonsei University College of Medicine (6-2021-0058), and a grant from the Korea Food Research Institute funded by the Ministry of Science, ICT & Future Planning (E0210400). Human stem cell line, CMC-hiPSC-011 was provided by National Stem Cell Bank of Korea (Korea National Institute of Health), originally provided from Catholic University.

Publisher Copyright:
© 2021 The Author(s)

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Developmental Biology
Cell Biology

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Lee, S. H., Oh, J., Lee, S. T., Won, D., Kim, S., Choi, H. K., Kim, S. J., Han, H., Yoon, M., Choi, J. R., Yoon, H. G., Park, S. W., Kang, S. M., & Lee, S. H. (2022). Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes. Stem Cell Research, 59, [102629]. https://doi.org/10.1016/j.scr.2021.102629

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title = "Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes",

abstract = "Dilated cardiomyopathy (DCM) is a heart muscle disease that causes heart failure and is the leading cause for heart transplantation. It is a heart muscle disease resulted from a variety of genetics, toxic, metabolic, and infectious causes. One of the most prevalent genetic causes of DCM is a protein-truncating variant in the Titin gene (TTNtv). We have generated a human-induced pluripotent stem cell (hiPSC) line from patients who underwent heart transplantation due to DCM carrying a TTNtv mutation (c.70051C > T, p.Arg23351Ter) at the age of 20. The generated hiPSCs showed normal karyotype (46, XY) and expression of pluripotency markers, and were differentiated towards cardiomyocytes successfully.",

author = "Lee, {Sun Ho} and Jaewon Oh and Lee, {Seung Tae} and Dongju Won and Sangwoo Kim and Choi, {Hyo Kyoung} and Kim, {Seok Jun} and Hyunho Han and Minjae Yoon and Choi, {Jong Rak} and Yoon, {Ho Geun} and Park, {Sahng Wook} and Kang, {Seok Min} and Lee, {Seung Hyun}",

note = "Funding Information: This work has supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2021R1I1A1A01060135, 2019R1C1C1002334, 2018R1D1A1B07049956). This study also was supported by Faculty research grant of Yonsei University College of Medicine (6-2021-0058), and a grant from the Korea Food Research Institute funded by the Ministry of Science, ICT & Future Planning (E0210400). Human stem cell line, CMC-hiPSC-011 was provided by National Stem Cell Bank of Korea (Korea National Institute of Health), originally provided from Catholic University. Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2022",

month = mar,

doi = "10.1016/j.scr.2021.102629",

language = "English",

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Lee, SH, Oh, J, Lee, ST, Won, D, Kim, S, Choi, HK, Kim, SJ, Han, H, Yoon, M, Choi, JR, Yoon, HG, Park, SW, Kang, SM & Lee, SH 2022, 'Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes', Stem Cell Research, vol. 59, 102629. https://doi.org/10.1016/j.scr.2021.102629

Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes. / Lee, Sun Ho; Oh, Jaewon; Lee, Seung Tae et al.

In: Stem Cell Research, Vol. 59, 102629, 03.2022.

Research output: Contribution to journal › Article › peer-review

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AU - Lee, Sun Ho

AU - Oh, Jaewon

AU - Lee, Seung Tae

AU - Won, Dongju

AU - Kim, Sangwoo

AU - Choi, Hyo Kyoung

AU - Kim, Seok Jun

AU - Han, Hyunho

AU - Yoon, Minjae

AU - Choi, Jong Rak

AU - Yoon, Ho Geun

AU - Park, Sahng Wook

AU - Kang, Seok Min

AU - Lee, Seung Hyun

N1 - Funding Information: This work has supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2021R1I1A1A01060135, 2019R1C1C1002334, 2018R1D1A1B07049956). This study also was supported by Faculty research grant of Yonsei University College of Medicine (6-2021-0058), and a grant from the Korea Food Research Institute funded by the Ministry of Science, ICT & Future Planning (E0210400). Human stem cell line, CMC-hiPSC-011 was provided by National Stem Cell Bank of Korea (Korea National Institute of Health), originally provided from Catholic University. Publisher Copyright: © 2021 The Author(s)

PY - 2022/3

Y1 - 2022/3

N2 - Dilated cardiomyopathy (DCM) is a heart muscle disease that causes heart failure and is the leading cause for heart transplantation. It is a heart muscle disease resulted from a variety of genetics, toxic, metabolic, and infectious causes. One of the most prevalent genetic causes of DCM is a protein-truncating variant in the Titin gene (TTNtv). We have generated a human-induced pluripotent stem cell (hiPSC) line from patients who underwent heart transplantation due to DCM carrying a TTNtv mutation (c.70051C > T, p.Arg23351Ter) at the age of 20. The generated hiPSCs showed normal karyotype (46, XY) and expression of pluripotency markers, and were differentiated towards cardiomyocytes successfully.

AB - Dilated cardiomyopathy (DCM) is a heart muscle disease that causes heart failure and is the leading cause for heart transplantation. It is a heart muscle disease resulted from a variety of genetics, toxic, metabolic, and infectious causes. One of the most prevalent genetic causes of DCM is a protein-truncating variant in the Titin gene (TTNtv). We have generated a human-induced pluripotent stem cell (hiPSC) line from patients who underwent heart transplantation due to DCM carrying a TTNtv mutation (c.70051C > T, p.Arg23351Ter) at the age of 20. The generated hiPSCs showed normal karyotype (46, XY) and expression of pluripotency markers, and were differentiated towards cardiomyocytes successfully.

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SN - 1873-5061

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Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes

Abstract

Bibliographical note

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UN SDGs

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