Abstract
Med28 plays a role in transcription, signal transduction, and cell proliferation. The overexpression of med28 is associated with tumor progression in in vitro and in vivo models. Recently it has been reported that the elevated expression of med28 is associated with poor outcome in women with breast cancer. The expression level of med28 in in vitro and in vivo was examined by using anti-rabbit polyclonal antibody in previous reports. In this study, we report for the first time the generation and characterization of four monoclonal antibodies against med28 through immunoblotting, immunofluorescence microscopy, immunoprecipitation, and immunohistochemical analyses. These antibodies will be useful in detecting med28 in in vitro and in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 30-35 |
| Number of pages | 6 |
| Journal | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy |
| Volume | 34 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2015 Feb 1 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
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}
Generation of Med28 Specific Monoclonal Antibodies. / Yu, Min A.; Cho, Jin Gu; Kim, Kwang Il; Jo, Yeong Joon; Sung, Jong Hyuk; Yang, Ho Bin; Park, Sang Gyu.
In: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, Vol. 34, No. 1, 01.02.2015, p. 30-35.Research output: Contribution to journal › Article
TY - JOUR
T1 - Generation of Med28 Specific Monoclonal Antibodies
AU - Yu, Min A.
AU - Cho, Jin Gu
AU - Kim, Kwang Il
AU - Jo, Yeong Joon
AU - Sung, Jong Hyuk
AU - Yang, Ho Bin
AU - Park, Sang Gyu
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Med28 plays a role in transcription, signal transduction, and cell proliferation. The overexpression of med28 is associated with tumor progression in in vitro and in vivo models. Recently it has been reported that the elevated expression of med28 is associated with poor outcome in women with breast cancer. The expression level of med28 in in vitro and in vivo was examined by using anti-rabbit polyclonal antibody in previous reports. In this study, we report for the first time the generation and characterization of four monoclonal antibodies against med28 through immunoblotting, immunofluorescence microscopy, immunoprecipitation, and immunohistochemical analyses. These antibodies will be useful in detecting med28 in in vitro and in vivo.
AB - Med28 plays a role in transcription, signal transduction, and cell proliferation. The overexpression of med28 is associated with tumor progression in in vitro and in vivo models. Recently it has been reported that the elevated expression of med28 is associated with poor outcome in women with breast cancer. The expression level of med28 in in vitro and in vivo was examined by using anti-rabbit polyclonal antibody in previous reports. In this study, we report for the first time the generation and characterization of four monoclonal antibodies against med28 through immunoblotting, immunofluorescence microscopy, immunoprecipitation, and immunohistochemical analyses. These antibodies will be useful in detecting med28 in in vitro and in vivo.
UR - http://www.scopus.com/inward/record.url?scp=84924423179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84924423179&partnerID=8YFLogxK
U2 - 10.1089/mab.2014.0068
DO - 10.1089/mab.2014.0068
M3 - Article
C2 - 25723281
AN - SCOPUS:84924423179
VL - 34
SP - 30
EP - 35
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
SN - 2167-9436
IS - 1
ER -