Generation of Med28 Specific Monoclonal Antibodies

Min A. Yu, Jin Gu Cho, Kwang Il Kim, Yeong Joon Jo, Jong Hyuk Sung, Ho Bin Yang, Sang Gyu Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Med28 plays a role in transcription, signal transduction, and cell proliferation. The overexpression of med28 is associated with tumor progression in in vitro and in vivo models. Recently it has been reported that the elevated expression of med28 is associated with poor outcome in women with breast cancer. The expression level of med28 in in vitro and in vivo was examined by using anti-rabbit polyclonal antibody in previous reports. In this study, we report for the first time the generation and characterization of four monoclonal antibodies against med28 through immunoblotting, immunofluorescence microscopy, immunoprecipitation, and immunohistochemical analyses. These antibodies will be useful in detecting med28 in in vitro and in vivo.

Original languageEnglish
Pages (from-to)30-35
Number of pages6
JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume34
Issue number1
DOIs
Publication statusPublished - 2015 Feb 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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