Generation of nitric oxide in the opossum lower esophageal sphincter during physiological experimentation

Se Joon Lee, Hyojin Park, Jin Hyuck Chang, Jeffrey L. Conklin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS), given in vivo, modulates opossum esophageal motor functions by inducing the inducible nitric oxide synthase (iNOS), which increases nitric oxide (NO) production. Superoxide, a NO scavenger, is generated during this endotoxemia. Superoxide is cleared by superoxide dismutase (SOD) and catalase (CAT) to protect the physiological function of NO. This study examined whether lower esophageal sphincter (LES) motility, NO release, and iNOS and nitrotyrosine accumulation in the LES are affected by LPS in vitro. Muscle strips from the opossum LES were placed in tissue baths containing oxygenated Krebs buffer. NO release was measured with a chemiluminescence NOx analyzer, and Western blots were performed to analyze iNOS and nitrotyrosine production. The percent change in resting LES tone after a 6-hour exposure to LPS was significantly increased compared to pretreatment values. The percent LES relaxation upon electrical stimulation was significantly decreased in the control group at 6 hours, indicating that the LPS treatment had an effect. The NO concentration in the tissue bath of LPS-treated muscle without nerve stimulation was significantly less than that of LPS treatment combined with SOD/CAT or SOD/CAT alone. iNOS and nitrotyrosine were detectable and increased over time in the LES muscle of both the control and LPS-treated groups. Antioxidant enzymes may play a role in regulating NO-mediated neuromuscular functions in the LES.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalYonsei medical journal
Volume47
Issue number2
DOIs
Publication statusPublished - 2006 Apr 1

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Opossums
Lower Esophageal Sphincter
Lipopolysaccharides
Nitric Oxide
Nitric Oxide Synthase Type II
Catalase
Superoxide Dismutase
Baths
Superoxides
Muscles
Endotoxemia
Luminescence
Electric Stimulation
Buffers
Antioxidants
Western Blotting
Control Groups
Enzymes
Therapeutics

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "Lipopolysaccharide (LPS), given in vivo, modulates opossum esophageal motor functions by inducing the inducible nitric oxide synthase (iNOS), which increases nitric oxide (NO) production. Superoxide, a NO scavenger, is generated during this endotoxemia. Superoxide is cleared by superoxide dismutase (SOD) and catalase (CAT) to protect the physiological function of NO. This study examined whether lower esophageal sphincter (LES) motility, NO release, and iNOS and nitrotyrosine accumulation in the LES are affected by LPS in vitro. Muscle strips from the opossum LES were placed in tissue baths containing oxygenated Krebs buffer. NO release was measured with a chemiluminescence NOx analyzer, and Western blots were performed to analyze iNOS and nitrotyrosine production. The percent change in resting LES tone after a 6-hour exposure to LPS was significantly increased compared to pretreatment values. The percent LES relaxation upon electrical stimulation was significantly decreased in the control group at 6 hours, indicating that the LPS treatment had an effect. The NO concentration in the tissue bath of LPS-treated muscle without nerve stimulation was significantly less than that of LPS treatment combined with SOD/CAT or SOD/CAT alone. iNOS and nitrotyrosine were detectable and increased over time in the LES muscle of both the control and LPS-treated groups. Antioxidant enzymes may play a role in regulating NO-mediated neuromuscular functions in the LES.",
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Generation of nitric oxide in the opossum lower esophageal sphincter during physiological experimentation. / Lee, Se Joon; Park, Hyojin; Chang, Jin Hyuck; Conklin, Jeffrey L.

In: Yonsei medical journal, Vol. 47, No. 2, 01.04.2006, p. 223-229.

Research output: Contribution to journalArticle

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