Abstract
This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.
| Original language | English |
|---|---|
| Pages (from-to) | 1164-1169 |
| Number of pages | 6 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 126 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2005 Nov 1 |
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All Science Journal Classification (ASJC) codes
- Ageing
- Developmental Biology
Cite this
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Generation of reversible Rb-knockdown mice. / Yoon, Sik Choi; Jae, Eun Lee; Cheong, Cheolho; Young, Hoon Sung; Eun, Young Yang; Chan, Bae Park; Song, Jaewhan; Sang, Chul Park; Lee, Han Woong.
In: Mechanisms of Ageing and Development, Vol. 126, No. 11, 01.11.2005, p. 1164-1169.Research output: Contribution to journal › Article
TY - JOUR
T1 - Generation of reversible Rb-knockdown mice
AU - Yoon, Sik Choi
AU - Jae, Eun Lee
AU - Cheong, Cheolho
AU - Young, Hoon Sung
AU - Eun, Young Yang
AU - Chan, Bae Park
AU - Song, Jaewhan
AU - Sang, Chul Park
AU - Lee, Han Woong
PY - 2005/11/1
Y1 - 2005/11/1
N2 - This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.
AB - This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.
UR - http://www.scopus.com/inward/record.url?scp=25144491970&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=25144491970&partnerID=8YFLogxK
U2 - 10.1016/j.mad.2005.06.002
DO - 10.1016/j.mad.2005.06.002
M3 - Article
C2 - 16087217
AN - SCOPUS:25144491970
VL - 126
SP - 1164
EP - 1169
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 11
ER -