Generation of reversible Rb-knockdown mice

Sik Choi Yoon, Eun Lee Jae, Cheolho Cheong, Hoon Sung Young, Young Yang Eun, Bae Park Chan, Jaewhan Song, Chul Park Sang, Han Woong Lee

Research output: Contribution to journalArticle

Abstract

This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.

Original languageEnglish
Pages (from-to)1164-1169
Number of pages6
JournalMechanisms of Ageing and Development
Volume126
Issue number11
DOIs
Publication statusPublished - 2005 Nov 1

Fingerprint

Cyclin E
Doxycycline
Transgenic Mice
Embryonic Structures
Fibroblasts
Phenotype
Genes
Ectopic Gene Expression

All Science Journal Classification (ASJC) codes

  • Ageing
  • Developmental Biology

Cite this

Yoon, S. C., Jae, E. L., Cheong, C., Young, H. S., Eun, Y. Y., Chan, B. P., ... Lee, H. W. (2005). Generation of reversible Rb-knockdown mice. Mechanisms of Ageing and Development, 126(11), 1164-1169. https://doi.org/10.1016/j.mad.2005.06.002
Yoon, Sik Choi ; Jae, Eun Lee ; Cheong, Cheolho ; Young, Hoon Sung ; Eun, Young Yang ; Chan, Bae Park ; Song, Jaewhan ; Sang, Chul Park ; Lee, Han Woong. / Generation of reversible Rb-knockdown mice. In: Mechanisms of Ageing and Development. 2005 ; Vol. 126, No. 11. pp. 1164-1169.
@article{dbe1df9ef13c48e6bebc2be1be5921dc,
title = "Generation of reversible Rb-knockdown mice",
abstract = "This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.",
author = "Yoon, {Sik Choi} and Jae, {Eun Lee} and Cheolho Cheong and Young, {Hoon Sung} and Eun, {Young Yang} and Chan, {Bae Park} and Jaewhan Song and Sang, {Chul Park} and Lee, {Han Woong}",
year = "2005",
month = "11",
day = "1",
doi = "10.1016/j.mad.2005.06.002",
language = "English",
volume = "126",
pages = "1164--1169",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier Ireland Ltd",
number = "11",

}

Yoon, SC, Jae, EL, Cheong, C, Young, HS, Eun, YY, Chan, BP, Song, J, Sang, CP & Lee, HW 2005, 'Generation of reversible Rb-knockdown mice', Mechanisms of Ageing and Development, vol. 126, no. 11, pp. 1164-1169. https://doi.org/10.1016/j.mad.2005.06.002

Generation of reversible Rb-knockdown mice. / Yoon, Sik Choi; Jae, Eun Lee; Cheong, Cheolho; Young, Hoon Sung; Eun, Young Yang; Chan, Bae Park; Song, Jaewhan; Sang, Chul Park; Lee, Han Woong.

In: Mechanisms of Ageing and Development, Vol. 126, No. 11, 01.11.2005, p. 1164-1169.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Generation of reversible Rb-knockdown mice

AU - Yoon, Sik Choi

AU - Jae, Eun Lee

AU - Cheong, Cheolho

AU - Young, Hoon Sung

AU - Eun, Young Yang

AU - Chan, Bae Park

AU - Song, Jaewhan

AU - Sang, Chul Park

AU - Lee, Han Woong

PY - 2005/11/1

Y1 - 2005/11/1

N2 - This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.

AB - This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.

UR - http://www.scopus.com/inward/record.url?scp=25144491970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25144491970&partnerID=8YFLogxK

U2 - 10.1016/j.mad.2005.06.002

DO - 10.1016/j.mad.2005.06.002

M3 - Article

C2 - 16087217

AN - SCOPUS:25144491970

VL - 126

SP - 1164

EP - 1169

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 11

ER -

Yoon SC, Jae EL, Cheong C, Young HS, Eun YY, Chan BP et al. Generation of reversible Rb-knockdown mice. Mechanisms of Ageing and Development. 2005 Nov 1;126(11):1164-1169. https://doi.org/10.1016/j.mad.2005.06.002