Generation of reversible Rb-knockdown mice

Sik Choi Yoon; Eun Lee Jae; Cheolho Cheong; Hoon Sung Young; Young Yang Eun; Bae Park Chan; Jaewhan Song; Chul Park Sang; Han Woong Lee

doi:10.1016/j.mad.2005.06.002

Generation of reversible Rb-knockdown mice

Sik Choi Yoon, Eun Lee Jae, Cheolho Cheong, Hoon Sung Young, Young Yang Eun, Bae Park Chan, Jaewhan Song, Chul Park Sang, Han Woong Lee

Research output: Contribution to journal › Article › peer-review

Abstract

This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb^-/- background were generated by crossings P_hCMV-tTA/TRE-Rb transgenic mice with conventional Rb ^+/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb ^-/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.

Original language	English
Pages (from-to)	1164-1169
Number of pages	6
Journal	Mechanisms of Ageing and Development
Volume	126
Issue number	11
DOIs	https://doi.org/10.1016/j.mad.2005.06.002
Publication status	Published - 2005 Nov

Bibliographical note

Funding Information:
This work was supported by the following grants: FG01-0304-002-1-0-0 of the 21C Frontier Functional Human Genome Project, M103KV010018-03K2201-01830 of BRC-Frontier from the Korean Ministry of Science and Technology and SRC (Molecular Therapy Research Center) grant from KOSEF.

All Science Journal Classification (ASJC) codes

Ageing
Developmental Biology

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10.1016/j.mad.2005.06.002

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title = "Generation of reversible Rb-knockdown mice",

abstract = "This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.",

author = "Yoon, {Sik Choi} and Jae, {Eun Lee} and Cheolho Cheong and Young, {Hoon Sung} and Eun, {Young Yang} and Chan, {Bae Park} and Jaewhan Song and Sang, {Chul Park} and Lee, {Han Woong}",

note = "Funding Information: This work was supported by the following grants: FG01-0304-002-1-0-0 of the 21C Frontier Functional Human Genome Project, M103KV010018-03K2201-01830 of BRC-Frontier from the Korean Ministry of Science and Technology and SRC (Molecular Therapy Research Center) grant from KOSEF.",

year = "2005",

month = nov,

doi = "10.1016/j.mad.2005.06.002",

language = "English",

volume = "126",

pages = "1164--1169",

journal = "Mechanisms of Ageing and Development",

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publisher = "Elsevier Ireland Ltd",

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T1 - Generation of reversible Rb-knockdown mice

AU - Yoon, Sik Choi

AU - Jae, Eun Lee

AU - Cheong, Cheolho

AU - Young, Hoon Sung

AU - Eun, Young Yang

AU - Chan, Bae Park

AU - Song, Jaewhan

AU - Sang, Chul Park

AU - Lee, Han Woong

N1 - Funding Information: This work was supported by the following grants: FG01-0304-002-1-0-0 of the 21C Frontier Functional Human Genome Project, M103KV010018-03K2201-01830 of BRC-Frontier from the Korean Ministry of Science and Technology and SRC (Molecular Therapy Research Center) grant from KOSEF.

PY - 2005/11

Y1 - 2005/11

N2 - This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.

AB - This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb +/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb -/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.

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JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 11

ER -

Generation of reversible Rb-knockdown mice

Abstract

Bibliographical note

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