Generation of the replication-competent human immunodeficiency virus type 1 which expresses a jellyfish green fluorescent protein

Ann Hwee Lee, Jung Min Han, Young Chul Sung

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The gene encoding a red-shifted green fluorescent protein variant (EGFP) was introduced into a human immunodeficiency virus type 1 (HIV-1) molecular clone by replacing the nef gene. The EGFP-expressing HIV-1 replicated efficiently in established human T cells. The expression of EGFP in the virus-infected cells was confirmed by fluorescence microscopy and also by Western blot analysis using the GFP antibodies. The EGFP gene was stably maintained in the viral genome during prolonged passages. EGFP-expressing HIV-1 could be used for anti-HIV-1 drug screening, sorting of virus-infected cells by fluorescence activated cell sorting (FACS) analysis, and for the rapid and simple detection of virus-infected cells by fluorescence microscopy.

Original languageEnglish
Pages (from-to)288-292
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume233
Issue number1
DOIs
Publication statusPublished - 1997 Apr 7

Fingerprint

Green Fluorescent Proteins
Viruses
HIV-1
Fluorescence Microscopy
nef Genes
Genes
Fluorescence microscopy
Preclinical Drug Evaluations
Viral Genome
Sorting
Flow Cytometry
Clone Cells
Western Blotting
Gene encoding
T-cells
T-Lymphocytes
Antibodies
Screening
Fluorescence
Cells

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Generation of the replication-competent human immunodeficiency virus type 1 which expresses a jellyfish green fluorescent protein. / Lee, Ann Hwee; Han, Jung Min; Sung, Young Chul.

In: Biochemical and Biophysical Research Communications, Vol. 233, No. 1, 07.04.1997, p. 288-292.

Research output: Contribution to journalArticle

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