Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Dasom Mun, Ji Young Kang, Yumin Chun, Da Seul Park, Hyoeun Kim, Nuri Yun, Seung Hyun Lee, Boyoung Joung

Research output: Contribution to journalArticlepeer-review

Abstract

PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and spontaneous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.

Original languageEnglish
Article number102940
JournalStem Cell Research
Volume65
DOIs
Publication statusPublished - 2022 Dec

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIT) (NRF-2021R1I1A1A01052197, NRF-2021R1C1C2094541); the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (KFRM21B0604L1-01); and the Korean Cardiac Research Foundation (202101-02).

Publisher Copyright:
© 2022 The Authors

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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