Genes are differentially expressed in the epididymal fat of rats rendered obese by a high-fat diet

Yun Jung Kim, Taesun Park

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The aim of present study was to identify the visceral adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity. Male Sprague-Dawley rats were fed either the HFD (17 g lard + 3 g corn oil/100 g) or the normal diet (5 g corn oil/100 g) for 9 weeks. The HFD rats weighed 55% more and accumulated 85% to 133% greater visceral fats than did the normal-diet rats (P < .05). Animals given the HFD for 9 weeks acquired dyslipidemia, fatty liver, insulin resistance, and hyperleptinemia along with the overexpression of several obesity-related genes, such as leptin, tumor necrosis factor α, resistin, peroxisome proliferator-activated receptor γ2, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein-1c, in the epididymal adipose tissue. The differential gene expression profile obtained from the cDNA microarray analysis followed by the real-time polymerase chain reaction confirmation led to a recruitment of several uncharacterized adipose tissue genes responding to the HFD. We report herein, for the first time, that a series of genes which might be implicated in the insulin-stimulated glucose transporter 4 translocation, such as protein phosphatase 2 (formerly 2A), cell division cycle 42-interacting protein 4, syntaxin 6, linker of T-cell receptor pathways 10, as well as the genes which might be involved in cancer development, such as heat shock 10-kd protein 1, and ras-related C3 botulinum toxin substrate 1, were differentially expressed in the epididymal adipose tissue of rats rendered obese by an HFD.

Original languageEnglish
Pages (from-to)414-422
Number of pages9
JournalNutrition Research
Volume28
Issue number6
DOIs
Publication statusPublished - 2008 Jun 1

Fingerprint

High Fat Diet
Fats
Genes
Adipose Tissue
Corn Oil
Intra-Abdominal Fat
rac1 GTP-Binding Protein
Chaperonin 10
Obesity
Qa-SNARE Proteins
Sterol Regulatory Element Binding Protein 1
CCAAT-Enhancer-Binding Proteins
Diet
Resistin
Protein Phosphatase 2
Peroxisome Proliferator-Activated Receptors
Facilitative Glucose Transport Proteins
Fatty Liver
Microarray Analysis
Dyslipidemias

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

Cite this

@article{f54b6664b2ab4771bec6f045a5f5a378,
title = "Genes are differentially expressed in the epididymal fat of rats rendered obese by a high-fat diet",
abstract = "The aim of present study was to identify the visceral adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity. Male Sprague-Dawley rats were fed either the HFD (17 g lard + 3 g corn oil/100 g) or the normal diet (5 g corn oil/100 g) for 9 weeks. The HFD rats weighed 55{\%} more and accumulated 85{\%} to 133{\%} greater visceral fats than did the normal-diet rats (P < .05). Animals given the HFD for 9 weeks acquired dyslipidemia, fatty liver, insulin resistance, and hyperleptinemia along with the overexpression of several obesity-related genes, such as leptin, tumor necrosis factor α, resistin, peroxisome proliferator-activated receptor γ2, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein-1c, in the epididymal adipose tissue. The differential gene expression profile obtained from the cDNA microarray analysis followed by the real-time polymerase chain reaction confirmation led to a recruitment of several uncharacterized adipose tissue genes responding to the HFD. We report herein, for the first time, that a series of genes which might be implicated in the insulin-stimulated glucose transporter 4 translocation, such as protein phosphatase 2 (formerly 2A), cell division cycle 42-interacting protein 4, syntaxin 6, linker of T-cell receptor pathways 10, as well as the genes which might be involved in cancer development, such as heat shock 10-kd protein 1, and ras-related C3 botulinum toxin substrate 1, were differentially expressed in the epididymal adipose tissue of rats rendered obese by an HFD.",
author = "Kim, {Yun Jung} and Taesun Park",
year = "2008",
month = "6",
day = "1",
doi = "10.1016/j.nutres.2008.03.015",
language = "English",
volume = "28",
pages = "414--422",
journal = "Nutrition Research",
issn = "0271-5317",
publisher = "Elsevier Inc.",
number = "6",

}

Genes are differentially expressed in the epididymal fat of rats rendered obese by a high-fat diet. / Kim, Yun Jung; Park, Taesun.

In: Nutrition Research, Vol. 28, No. 6, 01.06.2008, p. 414-422.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genes are differentially expressed in the epididymal fat of rats rendered obese by a high-fat diet

AU - Kim, Yun Jung

AU - Park, Taesun

PY - 2008/6/1

Y1 - 2008/6/1

N2 - The aim of present study was to identify the visceral adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity. Male Sprague-Dawley rats were fed either the HFD (17 g lard + 3 g corn oil/100 g) or the normal diet (5 g corn oil/100 g) for 9 weeks. The HFD rats weighed 55% more and accumulated 85% to 133% greater visceral fats than did the normal-diet rats (P < .05). Animals given the HFD for 9 weeks acquired dyslipidemia, fatty liver, insulin resistance, and hyperleptinemia along with the overexpression of several obesity-related genes, such as leptin, tumor necrosis factor α, resistin, peroxisome proliferator-activated receptor γ2, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein-1c, in the epididymal adipose tissue. The differential gene expression profile obtained from the cDNA microarray analysis followed by the real-time polymerase chain reaction confirmation led to a recruitment of several uncharacterized adipose tissue genes responding to the HFD. We report herein, for the first time, that a series of genes which might be implicated in the insulin-stimulated glucose transporter 4 translocation, such as protein phosphatase 2 (formerly 2A), cell division cycle 42-interacting protein 4, syntaxin 6, linker of T-cell receptor pathways 10, as well as the genes which might be involved in cancer development, such as heat shock 10-kd protein 1, and ras-related C3 botulinum toxin substrate 1, were differentially expressed in the epididymal adipose tissue of rats rendered obese by an HFD.

AB - The aim of present study was to identify the visceral adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity. Male Sprague-Dawley rats were fed either the HFD (17 g lard + 3 g corn oil/100 g) or the normal diet (5 g corn oil/100 g) for 9 weeks. The HFD rats weighed 55% more and accumulated 85% to 133% greater visceral fats than did the normal-diet rats (P < .05). Animals given the HFD for 9 weeks acquired dyslipidemia, fatty liver, insulin resistance, and hyperleptinemia along with the overexpression of several obesity-related genes, such as leptin, tumor necrosis factor α, resistin, peroxisome proliferator-activated receptor γ2, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein-1c, in the epididymal adipose tissue. The differential gene expression profile obtained from the cDNA microarray analysis followed by the real-time polymerase chain reaction confirmation led to a recruitment of several uncharacterized adipose tissue genes responding to the HFD. We report herein, for the first time, that a series of genes which might be implicated in the insulin-stimulated glucose transporter 4 translocation, such as protein phosphatase 2 (formerly 2A), cell division cycle 42-interacting protein 4, syntaxin 6, linker of T-cell receptor pathways 10, as well as the genes which might be involved in cancer development, such as heat shock 10-kd protein 1, and ras-related C3 botulinum toxin substrate 1, were differentially expressed in the epididymal adipose tissue of rats rendered obese by an HFD.

UR - http://www.scopus.com/inward/record.url?scp=44149086375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44149086375&partnerID=8YFLogxK

U2 - 10.1016/j.nutres.2008.03.015

DO - 10.1016/j.nutres.2008.03.015

M3 - Article

C2 - 19083440

AN - SCOPUS:44149086375

VL - 28

SP - 414

EP - 422

JO - Nutrition Research

JF - Nutrition Research

SN - 0271-5317

IS - 6

ER -