Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction

B. He, A. Shiau, Kang-Yell Choi, H. Zalkin, J. M. Smith

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Fusions of lacZ were constructed to genes in each of the loci involved in the novo synthesis of IMP. The expression of each pur-lacZ fusion was determined in isogenic purR and purR + strains. These measurements indicated 5- to 17-fold coregulation of genes purF, purHD, purC, purMN, purL, and purEK and thus confirm the existence of a pur regulon. Gene purB, which encodes an enzyme involved in synthesis of IMP and in the AMP branch of the pathway, was not regulated by purR. Each locus of the pur regulon contains a 16-base-pair conserved operator sequence that overlaps with the promoter. The purR product, purine repressor, was shown to bind specifically to each operator. Thus, binding of repressor to each operator of pur regulon genes negatively coregulates expression.

Original languageEnglish
Pages (from-to)4555-4562
Number of pages8
JournalJournal of Bacteriology
Volume172
Issue number8
Publication statusPublished - 1990 Aug 18

Fingerprint

Regulon
Escherichia coli
Inosine Monophosphate
Genes
Conserved Sequence
Adenosine Monophosphate
Base Pairing
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

@article{1b7f6af244884e8e963c5a26567e1606,
title = "Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction",
abstract = "Fusions of lacZ were constructed to genes in each of the loci involved in the novo synthesis of IMP. The expression of each pur-lacZ fusion was determined in isogenic purR and purR + strains. These measurements indicated 5- to 17-fold coregulation of genes purF, purHD, purC, purMN, purL, and purEK and thus confirm the existence of a pur regulon. Gene purB, which encodes an enzyme involved in synthesis of IMP and in the AMP branch of the pathway, was not regulated by purR. Each locus of the pur regulon contains a 16-base-pair conserved operator sequence that overlaps with the promoter. The purR product, purine repressor, was shown to bind specifically to each operator. Thus, binding of repressor to each operator of pur regulon genes negatively coregulates expression.",
author = "B. He and A. Shiau and Kang-Yell Choi and H. Zalkin and Smith, {J. M.}",
year = "1990",
month = "8",
day = "18",
language = "English",
volume = "172",
pages = "4555--4562",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "8",

}

Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction. / He, B.; Shiau, A.; Choi, Kang-Yell; Zalkin, H.; Smith, J. M.

In: Journal of Bacteriology, Vol. 172, No. 8, 18.08.1990, p. 4555-4562.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction

AU - He, B.

AU - Shiau, A.

AU - Choi, Kang-Yell

AU - Zalkin, H.

AU - Smith, J. M.

PY - 1990/8/18

Y1 - 1990/8/18

N2 - Fusions of lacZ were constructed to genes in each of the loci involved in the novo synthesis of IMP. The expression of each pur-lacZ fusion was determined in isogenic purR and purR + strains. These measurements indicated 5- to 17-fold coregulation of genes purF, purHD, purC, purMN, purL, and purEK and thus confirm the existence of a pur regulon. Gene purB, which encodes an enzyme involved in synthesis of IMP and in the AMP branch of the pathway, was not regulated by purR. Each locus of the pur regulon contains a 16-base-pair conserved operator sequence that overlaps with the promoter. The purR product, purine repressor, was shown to bind specifically to each operator. Thus, binding of repressor to each operator of pur regulon genes negatively coregulates expression.

AB - Fusions of lacZ were constructed to genes in each of the loci involved in the novo synthesis of IMP. The expression of each pur-lacZ fusion was determined in isogenic purR and purR + strains. These measurements indicated 5- to 17-fold coregulation of genes purF, purHD, purC, purMN, purL, and purEK and thus confirm the existence of a pur regulon. Gene purB, which encodes an enzyme involved in synthesis of IMP and in the AMP branch of the pathway, was not regulated by purR. Each locus of the pur regulon contains a 16-base-pair conserved operator sequence that overlaps with the promoter. The purR product, purine repressor, was shown to bind specifically to each operator. Thus, binding of repressor to each operator of pur regulon genes negatively coregulates expression.

UR - http://www.scopus.com/inward/record.url?scp=0025330317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025330317&partnerID=8YFLogxK

M3 - Article

VL - 172

SP - 4555

EP - 4562

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 8

ER -