Abstract
Cold-adapted live attenuated influenza vaccines (CAIVs) have been considered as a safe prophylactic measure to prevent influenza virus infections. The safety of a CAIV depends largely on genetic markers that confer specific attenuation phenotypes. Previous studies with other CAIVs reported that polymerase genes were primarily responsible for the attenuation. Here, we analyzed the genetic mutations and their phenotypic contribution in the X-31 ca strain, a recently developed alternative CAIV donor strain. During the cold-adaptation of its parental X-31 virus, various numbers of sequence changes were accumulated in all six internal genes. Phenotypic analysis with single-gene and multiple-gene reassortant viruses suggests that NP gene makes the largest contribution to the cold-adapted (ca) and temperature-sensitive (ts) characters, while the remaining other internal genes also impart attenuation characters with varying degrees. A balanced contribution of all internal genes to the attenuation suggests that X-31 ca could serve as an ideal master donor strain for CAIVs preventing influenza epidemics and pandemics.
| Original language | English |
|---|---|
| Pages (from-to) | 1343-1349 |
| Number of pages | 7 |
| Journal | Vaccine |
| Volume | 34 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2016 Mar 8 |
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All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Immunology and Microbiology(all)
- veterinary(all)
- Public Health, Environmental and Occupational Health
- Infectious Diseases
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Genetic analysis of attenuation markers of cold-adapted X-31 influenza live vaccine donor strain. / Jang, Yo Han; Jung, Eun Ju; Lee, Kwang Hee; Byun, Young Ho; Yang, Seung Won; Seong, Baik Lin.
In: Vaccine, Vol. 34, No. 11, 08.03.2016, p. 1343-1349.Research output: Contribution to journal › Article
TY - JOUR
T1 - Genetic analysis of attenuation markers of cold-adapted X-31 influenza live vaccine donor strain
AU - Jang, Yo Han
AU - Jung, Eun Ju
AU - Lee, Kwang Hee
AU - Byun, Young Ho
AU - Yang, Seung Won
AU - Seong, Baik Lin
PY - 2016/3/8
Y1 - 2016/3/8
N2 - Cold-adapted live attenuated influenza vaccines (CAIVs) have been considered as a safe prophylactic measure to prevent influenza virus infections. The safety of a CAIV depends largely on genetic markers that confer specific attenuation phenotypes. Previous studies with other CAIVs reported that polymerase genes were primarily responsible for the attenuation. Here, we analyzed the genetic mutations and their phenotypic contribution in the X-31 ca strain, a recently developed alternative CAIV donor strain. During the cold-adaptation of its parental X-31 virus, various numbers of sequence changes were accumulated in all six internal genes. Phenotypic analysis with single-gene and multiple-gene reassortant viruses suggests that NP gene makes the largest contribution to the cold-adapted (ca) and temperature-sensitive (ts) characters, while the remaining other internal genes also impart attenuation characters with varying degrees. A balanced contribution of all internal genes to the attenuation suggests that X-31 ca could serve as an ideal master donor strain for CAIVs preventing influenza epidemics and pandemics.
AB - Cold-adapted live attenuated influenza vaccines (CAIVs) have been considered as a safe prophylactic measure to prevent influenza virus infections. The safety of a CAIV depends largely on genetic markers that confer specific attenuation phenotypes. Previous studies with other CAIVs reported that polymerase genes were primarily responsible for the attenuation. Here, we analyzed the genetic mutations and their phenotypic contribution in the X-31 ca strain, a recently developed alternative CAIV donor strain. During the cold-adaptation of its parental X-31 virus, various numbers of sequence changes were accumulated in all six internal genes. Phenotypic analysis with single-gene and multiple-gene reassortant viruses suggests that NP gene makes the largest contribution to the cold-adapted (ca) and temperature-sensitive (ts) characters, while the remaining other internal genes also impart attenuation characters with varying degrees. A balanced contribution of all internal genes to the attenuation suggests that X-31 ca could serve as an ideal master donor strain for CAIVs preventing influenza epidemics and pandemics.
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U2 - 10.1016/j.vaccine.2016.01.053
DO - 10.1016/j.vaccine.2016.01.053
M3 - Article
VL - 34
SP - 1343
EP - 1349
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 11
ER -