Genetic analysis of liver metastatic cell lines with different metastatic potential

Hong Hua Sun, Hai Cui, Yong Min Jin, Xiong Cui, Long Zhen Piao, Song Nan Zhang, Hyuncheol Chung, Xiong Hu Shen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Metastasis is the major feature of malignant tumors that causes 90% of cancer deaths. Our laboratory has already established liver metastatic clones with YCC-16, isolated from the blood of a gastric cancer patient and expanded in vitro culture using a repeated orthotopic implantation method, and had reported biologic behaviour of the parental YCC-16, the orthotopic primary S1L0, and S1L1, S2L2 and S3L3 liver metastatic clones. Here, using these cell lines, we screened from chromosomal abnormalities using karyotype analysis and micro-CGH matching. There were 31 genes screened using PCA method which were functionally related to cell adhesion. Also, there were 23 genes selected which were related to the liver specific metastasis but excluded genes related to adhesion. There were 4 genes which demonstrated reduced or increased expression stepwise with passage. In conclusion, our results should contribute to exploring the mechanisms of liver metastasis by gastric cancer.

Original languageEnglish
Pages (from-to)3257-3263
Number of pages7
JournalAsian Pacific Journal of Cancer Prevention
Volume12
Issue number12
Publication statusPublished - 2011 Jan 1

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Cell Line
Liver
Neoplasm Metastasis
Genes
Stomach Neoplasms
Clone Cells
Passive Cutaneous Anaphylaxis
Karyotype
Cell Adhesion
Chromosome Aberrations
Neoplasms
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Sun, H. H., Cui, H., Jin, Y. M., Cui, X., Piao, L. Z., Zhang, S. N., ... Shen, X. H. (2011). Genetic analysis of liver metastatic cell lines with different metastatic potential. Asian Pacific Journal of Cancer Prevention, 12(12), 3257-3263.
Sun, Hong Hua ; Cui, Hai ; Jin, Yong Min ; Cui, Xiong ; Piao, Long Zhen ; Zhang, Song Nan ; Chung, Hyuncheol ; Shen, Xiong Hu. / Genetic analysis of liver metastatic cell lines with different metastatic potential. In: Asian Pacific Journal of Cancer Prevention. 2011 ; Vol. 12, No. 12. pp. 3257-3263.
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Sun, HH, Cui, H, Jin, YM, Cui, X, Piao, LZ, Zhang, SN, Chung, H & Shen, XH 2011, 'Genetic analysis of liver metastatic cell lines with different metastatic potential', Asian Pacific Journal of Cancer Prevention, vol. 12, no. 12, pp. 3257-3263.

Genetic analysis of liver metastatic cell lines with different metastatic potential. / Sun, Hong Hua; Cui, Hai; Jin, Yong Min; Cui, Xiong; Piao, Long Zhen; Zhang, Song Nan; Chung, Hyuncheol; Shen, Xiong Hu.

In: Asian Pacific Journal of Cancer Prevention, Vol. 12, No. 12, 01.01.2011, p. 3257-3263.

Research output: Contribution to journalArticle

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Sun HH, Cui H, Jin YM, Cui X, Piao LZ, Zhang SN et al. Genetic analysis of liver metastatic cell lines with different metastatic potential. Asian Pacific Journal of Cancer Prevention. 2011 Jan 1;12(12):3257-3263.