Genetic analysis of liver metastatic cell lines with different metastatic potential

Hong Hua Sun, Hai Cui, Yong Min Jin, Xiong Cui, Long Zhen Piao, Song Nan Zhang, Hyun Cheol Chung, Xiong Hu Shen

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Abstract

Metastasis is the major feature of malignant tumors that causes 90% of cancer deaths. Our laboratory has already established liver metastatic clones with YCC-16, isolated from the blood of a gastric cancer patient and expanded in vitro culture using a repeated orthotopic implantation method, and had reported biologic behaviour of the parental YCC-16, the orthotopic primary S1L0, and S1L1, S2L2 and S3L3 liver metastatic clones. Here, using these cell lines, we screened from chromosomal abnormalities using karyotype analysis and micro-CGH matching. There were 31 genes screened using PCA method which were functionally related to cell adhesion. Also, there were 23 genes selected which were related to the liver specific metastasis but excluded genes related to adhesion. There were 4 genes which demonstrated reduced or increased expression stepwise with passage. In conclusion, our results should contribute to exploring the mechanisms of liver metastasis by gastric cancer.

Original languageEnglish
Pages (from-to)3257-3263
Number of pages7
JournalAsian Pacific Journal of Cancer Prevention
Volume12
Issue number12
Publication statusPublished - 2011

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All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Sun, H. H., Cui, H., Jin, Y. M., Cui, X., Piao, L. Z., Zhang, S. N., Chung, H. C., & Shen, X. H. (2011). Genetic analysis of liver metastatic cell lines with different metastatic potential. Asian Pacific Journal of Cancer Prevention, 12(12), 3257-3263.