Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

Il Kwon Bae, You Nae Lee, Seok Hoon Jeong, Seong Geun Hong, Jung Hun Lee, Sang Hee Lee, Hyoung Jin Kim, Hasik Youn

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 β-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5-aac(6′)-IIa-blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most β-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4.

Original languageEnglish
Pages (from-to)465-468
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume58
Issue number4
DOIs
Publication statusPublished - 2007 Aug 1

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

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