Genetic comparison of stemness of human umbilical cord and dental pulp

Chung Min Kang, Hyunok Kim, Je Seon Song, Byung Jai Choi, Seong Oh Kim, Han Sung Jung, Seok Jun Moon, Hyung Jun Choi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study focuses on gene expression patterns and functions in human umbilical cord (UC) and dental pulp (DP) containing mesenchymal stem cells (MSCs). DP tissues were collected from 25 permanent premolars. UC tissue samples were obtained from three newborns. Comparative gene profiles were obtained using cDNA microarray analysis and the expression of tooth development-associated and MSC-related genes was assessed by the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Genes related to cell proliferation, angiogenesis, and immune responses were expressed at higher levels in UC, whereas genes related to growth factor and receptor activity and signal transduction were more highly expressed in DP. Although UC and DP tissues exhibited similar expression of surface markers for MSCs, UC showed higher expression of CD29, CD34, CD44, CD73, CD105, CD146, and CD166. qRT-PCR analysis showed that CD146, CD166, and MYC were expressed 18.3, 8.24, and 1.63 times more highly in UC, whereas the expression of CD34 was 2.15 times higher in DP. Immunohistochemical staining revealed significant differences in the expression of genes (DSPP, DMP1, and CALB1) related to odontogenesis and angiogenesis in DP. DP and UC tissue showed similar gene expression, with the usual MSC markers, while they clearly diverged in their differentiation capacity.

Original languageEnglish
Article number3453890
JournalStem Cells International
Volume2016
DOIs
Publication statusPublished - 2016 Jan 1

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Dental Pulp
Umbilical Cord
Mesenchymal Stromal Cells
Gene Expression
Genes
Reverse Transcription
Odontogenesis
Polymerase Chain Reaction
Growth Factor Receptors
Bicuspid
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Signal Transduction
Tooth
Cell Proliferation
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Kang, Chung Min ; Kim, Hyunok ; Song, Je Seon ; Choi, Byung Jai ; Kim, Seong Oh ; Jung, Han Sung ; Moon, Seok Jun ; Choi, Hyung Jun. / Genetic comparison of stemness of human umbilical cord and dental pulp. In: Stem Cells International. 2016 ; Vol. 2016.
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Genetic comparison of stemness of human umbilical cord and dental pulp. / Kang, Chung Min; Kim, Hyunok; Song, Je Seon; Choi, Byung Jai; Kim, Seong Oh; Jung, Han Sung; Moon, Seok Jun; Choi, Hyung Jun.

In: Stem Cells International, Vol. 2016, 3453890, 01.01.2016.

Research output: Contribution to journalArticle

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