Genetic dissection of naturally occurring basal core promoter mutations of hepatitis B virus reveals a silent phenotype in the overlapping X gene

Zahid Hussain, Hyeon Sik Jung, Dong Kyun Ryu, Wang-Shick Ryu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

During chronic hepatitis B virus (HBV) infection, double substitution mutations in the basal core promoter (BCP) region frequently emerge that include A1762T/G1764A and the neighbouring C1766T/T1768A mutations, here termed BCP1 and BCP2, respectively. Due to a compact viral genome organization, BCP1 and BCP2 mutations result in amino acids changes in the overlapping X gene: K130M/V131I and F132Y, respectively. It has been shown that both BCP mutations lead to a modest increase in viral genome replication. However, the question of whether the alteration that occurs in the overlapping X gene might contribute to the increased viral genome replication has not been properly addressed. This study genetically separated the core promoter from the overlapping X gene using 1.3mer overlength HBV constructs and examined the impact of the X gene mutations on viral genome replication in HepG2 cells. Each BCP mutation resulted in modestly enhanced viral genome replication that occurred via augmented viral transcription. Therefore, it was concluded that these BCP mutations do not affect expression of the overlapping X gene or impair its stimulatory effect on viral genome replication.

Original languageEnglish
Pages (from-to)2272-2281
Number of pages10
JournalJournal of General Virology
Volume90
Issue number9
DOIs
Publication statusPublished - 2009 Oct 5

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Overlapping Genes
Viral Genome
Hepatitis B virus
Dissection
Phenotype
Mutation
Chronic Hepatitis B
Hep G2 Cells
Virus Diseases
Genetic Promoter Regions
Amino Acids

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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abstract = "During chronic hepatitis B virus (HBV) infection, double substitution mutations in the basal core promoter (BCP) region frequently emerge that include A1762T/G1764A and the neighbouring C1766T/T1768A mutations, here termed BCP1 and BCP2, respectively. Due to a compact viral genome organization, BCP1 and BCP2 mutations result in amino acids changes in the overlapping X gene: K130M/V131I and F132Y, respectively. It has been shown that both BCP mutations lead to a modest increase in viral genome replication. However, the question of whether the alteration that occurs in the overlapping X gene might contribute to the increased viral genome replication has not been properly addressed. This study genetically separated the core promoter from the overlapping X gene using 1.3mer overlength HBV constructs and examined the impact of the X gene mutations on viral genome replication in HepG2 cells. Each BCP mutation resulted in modestly enhanced viral genome replication that occurred via augmented viral transcription. Therefore, it was concluded that these BCP mutations do not affect expression of the overlapping X gene or impair its stimulatory effect on viral genome replication.",
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Genetic dissection of naturally occurring basal core promoter mutations of hepatitis B virus reveals a silent phenotype in the overlapping X gene. / Hussain, Zahid; Jung, Hyeon Sik; Ryu, Dong Kyun; Ryu, Wang-Shick.

In: Journal of General Virology, Vol. 90, No. 9, 05.10.2009, p. 2272-2281.

Research output: Contribution to journalArticle

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