Genetic diversity among Korean Candida albicans bloodstream isolates: Assessment by multilocus sequence typing and restriction endonuclease analysis of genomic DNA by use of BssHII

Jong Hee Shin, Marie Elisabeth Bougnoux, Christophe D'Enfert, Soo Hyun Kim, Chang Jin Moon, Min Young Joo, Kyungwon Lee, Mi Na Kim, Hye Soo Lee, Myung Geun Shin, Soon Pal Suh, Dong Wook Ryang

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Multilocus sequence typing (MLST) has been successfully applied to the epidemiology of Candida albicans isolates not only within the hospital setting but also in multiple locations nationwide. We performed MLST to investigate the genetic relatedness among bloodstream infection (BSI) isolates of C. albicans recovered from 10 Korean hospitals over a 12-month period. The 156 isolates yielded 112 unique diploid sequence types (DSTs). While 95 DSTs were each derived from a single isolate, 17 DSTs were shared by 61 isolates (39.1%). Interestingly, 111 (71.1%) isolates clustered within previously known clades, and 29 (18.6%) clustered within a new clade that includes strains of Asian origin previously typed as singletons. This MLST study was complemented by restriction endonuclease analysis of genomic DNA using BssHII (REAG-B) in order to evaluate whether strains with identical DSTs and originating from the same hospital corresponded to nosocomial clusters. Importantly, only those isolates with a strong epidemiological relationship showed ≥95% identical REAG-B types. Our results indicate that REAG-B typing can be complementary to MLST but should be limited to the investigation of isolates of identical DSTs and when interhuman transmission is suspected.

Original languageEnglish
Pages (from-to)2572-2577
Number of pages6
JournalJournal of Clinical Microbiology
Volume49
Issue number7
DOIs
Publication statusPublished - 2011 Jul 1

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Multilocus Sequence Typing
DNA Restriction Enzymes
Diploidy
Candida albicans
DNA
Epidemiology
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Shin, Jong Hee ; Bougnoux, Marie Elisabeth ; D'Enfert, Christophe ; Kim, Soo Hyun ; Moon, Chang Jin ; Joo, Min Young ; Lee, Kyungwon ; Kim, Mi Na ; Lee, Hye Soo ; Shin, Myung Geun ; Suh, Soon Pal ; Ryang, Dong Wook. / Genetic diversity among Korean Candida albicans bloodstream isolates : Assessment by multilocus sequence typing and restriction endonuclease analysis of genomic DNA by use of BssHII. In: Journal of Clinical Microbiology. 2011 ; Vol. 49, No. 7. pp. 2572-2577.
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Genetic diversity among Korean Candida albicans bloodstream isolates : Assessment by multilocus sequence typing and restriction endonuclease analysis of genomic DNA by use of BssHII. / Shin, Jong Hee; Bougnoux, Marie Elisabeth; D'Enfert, Christophe; Kim, Soo Hyun; Moon, Chang Jin; Joo, Min Young; Lee, Kyungwon; Kim, Mi Na; Lee, Hye Soo; Shin, Myung Geun; Suh, Soon Pal; Ryang, Dong Wook.

In: Journal of Clinical Microbiology, Vol. 49, No. 7, 01.07.2011, p. 2572-2577.

Research output: Contribution to journalArticle

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AU - Ryang, Dong Wook

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