Genetic diversity of chromosomal metallo-β-lactamase genes in clinical isolates of Elizabethkingia meningoseptica from Korea

Jong Hwa Yum, Eun Young Lee, Sung Ho Hur, Seok Hoon Jeong, Hyukmin Lee, Dongeun Yong, Yunsop Chong, Eun Woo Lee, Patrice Nordmann, Kyungwon Lee

Research output: Contribution to journalArticle

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Abstract

This study was performed to characterize the chromosomal metallo-β-lactamases (MBLs) of Elizabethkingia meningoseptica isolated from Korea and to propose a clustering method of BlaB and GOB MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E. meningoseptica. These PCR products were then cloned into a vector and electrotransformed into E. coli DH5α. Nucleotide sequencing was performed by the dideoxy chain termination method using PCR products or cloned DNA fragments. Antimicrobial susceptibilities were determined by the agar dilution method. PCR experiments showed that all 31 E. meningoseptica isolates contained both the blaB and the blaGOB genes. DNA sequence analysis revealed that E. meningoseptica isolates possessed seven types of blaB gene, including five novel variants (blaB-9 to blaB-13) and 11 types of blaGOB gene, including 10 novel variants (blaGOB-8 to blaGOB-17). The most common combination of MBL was BlaB-12 plus GOB-17 (n=19). Minimum inhibitory concentrations of imipenem and meropenem for the electrotransformants harboring novel BlaB and GOB MBLs were two- or four-fold higher than those for the recipient E. coli DH5α. BlaB and GOB MBLs were grouped in three and six clusters including fifteen novel variants, respectively, based on amino acid similarities.

Original languageEnglish
Pages (from-to)358-364
Number of pages7
JournalJournal of Microbiology
Volume48
Issue number3
DOIs
Publication statusPublished - 2010 Jul 1

Fingerprint

Korea
Polymerase Chain Reaction
meropenem
Genes
Escherichia coli
Amino Acids
Imipenem
Microbial Sensitivity Tests
DNA Sequence Analysis
Agar
Cluster Analysis
Nucleotides
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Yum, Jong Hwa ; Lee, Eun Young ; Hur, Sung Ho ; Jeong, Seok Hoon ; Lee, Hyukmin ; Yong, Dongeun ; Chong, Yunsop ; Lee, Eun Woo ; Nordmann, Patrice ; Lee, Kyungwon. / Genetic diversity of chromosomal metallo-β-lactamase genes in clinical isolates of Elizabethkingia meningoseptica from Korea. In: Journal of Microbiology. 2010 ; Vol. 48, No. 3. pp. 358-364.
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Genetic diversity of chromosomal metallo-β-lactamase genes in clinical isolates of Elizabethkingia meningoseptica from Korea. / Yum, Jong Hwa; Lee, Eun Young; Hur, Sung Ho; Jeong, Seok Hoon; Lee, Hyukmin; Yong, Dongeun; Chong, Yunsop; Lee, Eun Woo; Nordmann, Patrice; Lee, Kyungwon.

In: Journal of Microbiology, Vol. 48, No. 3, 01.07.2010, p. 358-364.

Research output: Contribution to journalArticle

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