TY - JOUR
T1 - Genetic diversity of Schistosoma haematobium eggs isolated from human Urine in Sudan
AU - Quan, Juan Hua
AU - Choi, In Wook
AU - Ismail, Hassan Ahmed Hassan Ahmed
AU - Mohamed, Abdoelohab Saed
AU - Jeong, Hoo Gn
AU - Lee, Jin Su
AU - Hong, Sung Tae
AU - Yong, Tai Soon
AU - Cha, Guang Ho
AU - Lee, Young Ha
N1 - Publisher Copyright:
© 2015, Korean Society for Parasitology and Tropical Medicine.
PY - 2015/6/30
Y1 - 2015/6/30
N2 - The genetic diversity of Schistosoma haematobium remains largely unstudied in comparison to that of Schistosoma mansoni. To characterize the extent of genetic diversity in S. haematobium among its definitive host (humans), we collected S. haematobium eggs from the urine of 73 infected schoolchildren at 5 primary schools in White Nile State, Sudan, and then performed a randomly amplified polymorphic DNA marker ITS2 by PCR-RFLP analysis. Among 73 S. haematobium egg-positive cases, 13 were selected based on the presence of the S. haematobium satellite markers A4 and B2 in their genomic DNA, and used for RFLP analysis. The 13 samples were subjected to an RFLP analysis of the S. haematobium ITS2 region; however, there was no variation in size among the fragments. Compared to the ITS2 sequences obtained for S. haematobium from Kenya, the nucleotide sequences of the ITS2 regions of S. haematobium from 4 areas in Sudan were consistent with those from Kenya (> 99%). In this study, we demonstrate for the first time that most of the S. haematobium population in Sudan consists of a pan-African S. haematobium genotype; however, we also report the discovery of Kenyan strain inflow into White Nile, Sudan.
AB - The genetic diversity of Schistosoma haematobium remains largely unstudied in comparison to that of Schistosoma mansoni. To characterize the extent of genetic diversity in S. haematobium among its definitive host (humans), we collected S. haematobium eggs from the urine of 73 infected schoolchildren at 5 primary schools in White Nile State, Sudan, and then performed a randomly amplified polymorphic DNA marker ITS2 by PCR-RFLP analysis. Among 73 S. haematobium egg-positive cases, 13 were selected based on the presence of the S. haematobium satellite markers A4 and B2 in their genomic DNA, and used for RFLP analysis. The 13 samples were subjected to an RFLP analysis of the S. haematobium ITS2 region; however, there was no variation in size among the fragments. Compared to the ITS2 sequences obtained for S. haematobium from Kenya, the nucleotide sequences of the ITS2 regions of S. haematobium from 4 areas in Sudan were consistent with those from Kenya (> 99%). In this study, we demonstrate for the first time that most of the S. haematobium population in Sudan consists of a pan-African S. haematobium genotype; however, we also report the discovery of Kenyan strain inflow into White Nile, Sudan.
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U2 - 10.3347/kjp.2015.53.3.271
DO - 10.3347/kjp.2015.53.3.271
M3 - Article
C2 - 26174820
AN - SCOPUS:84937700879
VL - 53
SP - 271
EP - 277
JO - Korean Journal of Parasitology
JF - Korean Journal of Parasitology
SN - 0023-4001
IS - 3
ER -