Genome engineering has been developed to create useful strains for biological studies and industrial uses. However, a continuous challenge remained in the field: technical limitations in high-throughput screening and precise manipulation of strains. Today, technical improvements have made genome engineering more rapid and efficient. This review introduces recent advances in genome engineering technologies applied to Escherichia coli as well as multiplex automated genome engineering (MAGE), a recent technique proposed as a powerful toolkit due to its straightforward process, rapid experimental procedures, and highly efficient properties.
Bibliographical noteFunding Information:
The authors thank Hee Jin Youn for her helpful discussions and manuscript comments. This work was supported by the Next-Generation BioGreen 21 Program (grant no. PJ009034012013 ) and the Intelligent Synthetic Biology Center of the Global Frontier Project, funded by the Ministry of Science, ICT & Future Planning, Korea (grant no. 2011-0031956 ). Jaehwan Jeong was supported by a National Junior Research Fellowship from the National Research Foundation of Korea (grant no. 2011-0012414 ).
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology