Genome-scale promoter engineering by coselection MAGE

Harris H. Wang, Hwangbeom Kim, Le Cong, Jaehwan Jeong, Duhee Bang, George M. Church

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Multiplex automated genome engineering (MAGE) uses short oligonucleotides to scarlessly modify genomes; however, insertions >10 bases are still inefficient but can be improved substantially by selection of highly modified chromosomes. Here we describe 'coselection' MAGE (CoS-MAGE) to optimize biosynthesis of aromatic amino acid derivatives by combinatorially inserting multiple T7 promoters simultaneously into 12 genomic operons. Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks.

Original languageEnglish
Pages (from-to)591-593
Number of pages3
JournalNature Methods
Volume9
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1

Fingerprint

Genes
Genome
Aromatic Amino Acids
Gene Regulatory Networks
Operon
Oligonucleotides
Libraries
Biosynthesis
Chromosomes
Derivatives

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Wang, H. H., Kim, H., Cong, L., Jeong, J., Bang, D., & Church, G. M. (2012). Genome-scale promoter engineering by coselection MAGE. Nature Methods, 9(6), 591-593. https://doi.org/10.1038/nmeth.1971
Wang, Harris H. ; Kim, Hwangbeom ; Cong, Le ; Jeong, Jaehwan ; Bang, Duhee ; Church, George M. / Genome-scale promoter engineering by coselection MAGE. In: Nature Methods. 2012 ; Vol. 9, No. 6. pp. 591-593.
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Wang, HH, Kim, H, Cong, L, Jeong, J, Bang, D & Church, GM 2012, 'Genome-scale promoter engineering by coselection MAGE', Nature Methods, vol. 9, no. 6, pp. 591-593. https://doi.org/10.1038/nmeth.1971

Genome-scale promoter engineering by coselection MAGE. / Wang, Harris H.; Kim, Hwangbeom; Cong, Le; Jeong, Jaehwan; Bang, Duhee; Church, George M.

In: Nature Methods, Vol. 9, No. 6, 01.06.2012, p. 591-593.

Research output: Contribution to journalArticle

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Wang HH, Kim H, Cong L, Jeong J, Bang D, Church GM. Genome-scale promoter engineering by coselection MAGE. Nature Methods. 2012 Jun 1;9(6):591-593. https://doi.org/10.1038/nmeth.1971