Genome-scale promoter engineering by coselection MAGE

Harris H. Wang, Hwangbeom Kim, Le Cong, Jaehwan Jeong, Duhee Bang, George M. Church

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Multiplex automated genome engineering (MAGE) uses short oligonucleotides to scarlessly modify genomes; however, insertions >10 bases are still inefficient but can be improved substantially by selection of highly modified chromosomes. Here we describe 'coselection' MAGE (CoS-MAGE) to optimize biosynthesis of aromatic amino acid derivatives by combinatorially inserting multiple T7 promoters simultaneously into 12 genomic operons. Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks.

Original languageEnglish
Pages (from-to)591-593
Number of pages3
JournalNature Methods
Volume9
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Wang, H. H., Kim, H., Cong, L., Jeong, J., Bang, D., & Church, G. M. (2012). Genome-scale promoter engineering by coselection MAGE. Nature Methods, 9(6), 591-593. https://doi.org/10.1038/nmeth.1971