Genome sequence of Escherichia coli NCCP15653, a group D strain isolated from a diarrhea patient

Min Jung Kwak, Myung Soo Kim, Soon Kyeong Kwon, Seung Hak Cho, Jihyun F. Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Pathogenic strains in Escherichia coli can be divided into several pathotypes according to their virulence features. Among them, uropathogenic E. coli causes most of the urinary tract infections and has a genotype distinct from other virulent strains of E. coli. In this study, we sequenced and analyzed the genome of E. coli NCCP15653 isolated from the feces of a diarrhea patient in 2007 in South Korea. Results: A phylogenetic tree based on MLST showed that NCCP15653 belongs to the D group of E. coli and located in the lineage containing strains ST2747, UMN026 and 042. In the genome of NCCP15653, genes encoding major virulence factors of uropathogenic E. coli were detected. They include type I fimbriae, hemin uptake proteins, iron/manganese transport proteins, yersiniabactin siderophore proteins, type VI secretion proteins, and hemolysin. On the other hand, genes encoding AslA, OmpA, and the K1 capsule, which are virulence factors associated with invasion of neonatal meningitis-causing E. coli, were also present, while a gene encoding CNF-1 protein, which is a cytotoxic necrotizing factor 1, was not detected. Conclusions: Through the genome analysis of NCCP15653, we report an example of a genome of chimeric pathogenic properties. The gene content of NCCP15653, a group D strain, demonstrates that it could be both uropathogenic E. coli and neonatal meningitis-causing E. coli. Our results suggest the dynamic nature of plastic genomes in pathogenic strains of E. coli.

Original languageEnglish
Article number84
JournalGut Pathogens
Volume8
Issue number1
DOIs
Publication statusPublished - 2016 Feb 23

Fingerprint

Uropathogenic Escherichia coli
Diarrhea
Genome
Escherichia coli
Escherichia coli Meningitis
Virulence Factors
Genes
Siderophores
Republic of Korea
Hemin
Proteins
Hemolysin Proteins
Manganese
Urinary Tract Infections
Feces
Plastics
Capsules
Virulence
Carrier Proteins
Iron

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Gastroenterology
  • Virology
  • Infectious Diseases

Cite this

Kwak, Min Jung ; Kim, Myung Soo ; Kwon, Soon Kyeong ; Cho, Seung Hak ; Kim, Jihyun F. / Genome sequence of Escherichia coli NCCP15653, a group D strain isolated from a diarrhea patient. In: Gut Pathogens. 2016 ; Vol. 8, No. 1.
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Genome sequence of Escherichia coli NCCP15653, a group D strain isolated from a diarrhea patient. / Kwak, Min Jung; Kim, Myung Soo; Kwon, Soon Kyeong; Cho, Seung Hak; Kim, Jihyun F.

In: Gut Pathogens, Vol. 8, No. 1, 84, 23.02.2016.

Research output: Contribution to journalArticle

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AB - Background: Pathogenic strains in Escherichia coli can be divided into several pathotypes according to their virulence features. Among them, uropathogenic E. coli causes most of the urinary tract infections and has a genotype distinct from other virulent strains of E. coli. In this study, we sequenced and analyzed the genome of E. coli NCCP15653 isolated from the feces of a diarrhea patient in 2007 in South Korea. Results: A phylogenetic tree based on MLST showed that NCCP15653 belongs to the D group of E. coli and located in the lineage containing strains ST2747, UMN026 and 042. In the genome of NCCP15653, genes encoding major virulence factors of uropathogenic E. coli were detected. They include type I fimbriae, hemin uptake proteins, iron/manganese transport proteins, yersiniabactin siderophore proteins, type VI secretion proteins, and hemolysin. On the other hand, genes encoding AslA, OmpA, and the K1 capsule, which are virulence factors associated with invasion of neonatal meningitis-causing E. coli, were also present, while a gene encoding CNF-1 protein, which is a cytotoxic necrotizing factor 1, was not detected. Conclusions: Through the genome analysis of NCCP15653, we report an example of a genome of chimeric pathogenic properties. The gene content of NCCP15653, a group D strain, demonstrates that it could be both uropathogenic E. coli and neonatal meningitis-causing E. coli. Our results suggest the dynamic nature of plastic genomes in pathogenic strains of E. coli.

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