Background: Pathogenic strains in Escherichia coli can be divided into several pathotypes according to their virulence features. Among them, uropathogenic E. coli causes most of the urinary tract infections and has a genotype distinct from other virulent strains of E. coli. In this study, we sequenced and analyzed the genome of E. coli NCCP15653 isolated from the feces of a diarrhea patient in 2007 in South Korea. Results: A phylogenetic tree based on MLST showed that NCCP15653 belongs to the D group of E. coli and located in the lineage containing strains ST2747, UMN026 and 042. In the genome of NCCP15653, genes encoding major virulence factors of uropathogenic E. coli were detected. They include type I fimbriae, hemin uptake proteins, iron/manganese transport proteins, yersiniabactin siderophore proteins, type VI secretion proteins, and hemolysin. On the other hand, genes encoding AslA, OmpA, and the K1 capsule, which are virulence factors associated with invasion of neonatal meningitis-causing E. coli, were also present, while a gene encoding CNF-1 protein, which is a cytotoxic necrotizing factor 1, was not detected. Conclusions: Through the genome analysis of NCCP15653, we report an example of a genome of chimeric pathogenic properties. The gene content of NCCP15653, a group D strain, demonstrates that it could be both uropathogenic E. coli and neonatal meningitis-causing E. coli. Our results suggest the dynamic nature of plastic genomes in pathogenic strains of E. coli.
Bibliographical noteFunding Information:
The authors are thankful to Byung Kwon Kim, Ju Yeon Song, Seon‑Young Kim, and the KRIBB sequencing team for technical assistance. This work was finan‑ cially supported by the National Research Foundation of the Ministry of Sci‑ ence, ICT and Future Planning (NRF‑2011‑0017670 to J.F.K.) and Korea National Institute of Health (KNIH 4800‑4845‑300 to S.H.C.), Republic of Korea.
© 2016 Kwak et al.
All Science Journal Classification (ASJC) codes
- Infectious Diseases