Genome-wide screening and identification of factors affecting the biosynthesis of prodigiosin by Hahella chejuensis, using Escherichia coli as a surrogate host

Soon Kyeong Kwon, Yon Kyoung Park, Jihyun F. Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A marine bacterium, Hahella chejuensis, recently has attracted attention due to its lytic activity against a red-tide dinoflagellate. The algicidal function originates from its red pigment, prodigiosin, which also exhibits immunosuppressive or anticancer activity. Genome sequencing and functional analysis revealed a gene set contained in the hap gene cluster that is responsible for the biosynthesis of prodigiosin. To screen for the factors affecting the prodigiosin biosynthesis, we constructed a plasmid library of the H. chejuensis genomic DNA, introduced it into Escherichia coli strains harboring the hap cluster, and observed changes in production of the red pigment. Among the screened clones, hapXY genes whose products constitute a two-component signal transduction system were elucidated as positive regulators of the pigment production. In addition, an Hfqdependent, noncoding region located at one end of the hap cluster was confirmed to play roles in regulation. Identification of factors involved in the regulation of prodigiosin biosynthesis should help in understanding how the prodigiosin-biosynthetic pathway is organized and controlled and also aid in modulating the overexpression of prodigiosin in a heterologous host, such as E. coli, or in the natural producer, H. chejuensis.

Original languageEnglish
Pages (from-to)1661-1668
Number of pages8
JournalApplied and Environmental Microbiology
Volume76
Issue number5
DOIs
Publication statusPublished - 2010 Mar 1

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Hahella chejuensis
Prodigiosin
pigment
genome
pigments
biosynthesis
Genome
Escherichia coli
screening
gene
immunosuppressive agents
red tide
plasmid
multigene family
algal blooms
dinoflagellate
signal transduction
biochemical pathways
clone
genomics

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

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