Genomic organization and characterization of the promoter of rat malonyl-CoA decarboxylase gene

Gha Young Lee, Jin Won Cho, Hyun Chul Lee, Yu Sam Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Malonyl-CoA decarboxylase (MCD) catalyzes the decarboxylation of malonyl-CoA, an elongating agent for fatty acid synthesis and also known as a fuel-sensing mediator. In order to elucidate the genome organization, we isolated a 2020 bp rat MCD cDNA from rat brain cDNA library and isolated the corresponding rat genomic clones from the rat genomic PAC library. Sequencing and comparison of these clones showed that the MCD genome consists of five exons and four introns spanning approximately 17 kb. The proximal upstream region is GC-rich, lacks a TATA box, and contains a variety of putative transcriptional regulatory elements within 2 kb. A major transcriptional initiation site was identified by a primer extension at a site 157 nucleotides upstream of the translational initiation site. To investigate the transcriptional regulation of MCD, a series of 5′-deletion constructs of the 5′-flanking region were generated and cloned upstream from the luciferase reporter gene. By comparing promoter activity in CV-1 cells, we suggest that an area of -15 bp 5′ from the first exon acted as a basal promoter for MCD and that there are positive cis-regulatory elements in the region from -55 to -325 bp and negative regulator elements in the region -1380 to -2240 bp.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1577
Issue number1
DOIs
Publication statusPublished - 2002 Aug 19

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant (KRF-2000-015-DP0288) and partly by Korea Science and Engineering Foundation through the Protein Network Research Center at Yonsei University (R11-2000-006101-0). We thank Miss Min Sun Park for her assistance.

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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