Genomic structure of a double-stranded RNA virus in the parasite Leishmania major

Jae Myoung Suh, Gun Eui Lee, Young Min Kim, Joo Hun Lee, Dae Myoung Kim, Joo Won Suh, In Kwon Chung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Viruses have been isolated in Leishmania major, which is an old world strain of the protozoan parasite Leishmania. The complete nucleotide sequence of these viruses have recently been determined and named as LRV 2-1. These viral particles can contain either a double-stranded RNA or a single-stranded RNA genome. The ssRNA particles and dsRNA particles have different sedimentation properties on centrifugation through a sucrose gradient. Northern hybridization of fractions collected from the gradient to a riboprobe of known polarity shows that the ssRNA is of positive-sense polarity. Analysis of the two different RNA species under denaturing conditions shows that they are both linear and of genome length size. The 5′-ends of the viral genome do not contain any modified structures. Although a considerate amount of sequence divergence exists between LRV 2-1 and previously described LRV 1, a virus isolated in a new world strain of Leishmania, the basic genome structure is largely similar.

Original languageEnglish
Pages (from-to)40-44
Number of pages5
JournalMolecules and Cells
Volume6
Issue number1
Publication statusPublished - 1996 Feb 29

Fingerprint

Leishmania major
Double-Stranded RNA
RNA Viruses
Parasites
Leishmania
Viruses
Genome
RNA
Genome Size
Viral Genome
Centrifugation
Virion
Sucrose

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Suh, Jae Myoung ; Lee, Gun Eui ; Kim, Young Min ; Lee, Joo Hun ; Kim, Dae Myoung ; Suh, Joo Won ; Chung, In Kwon. / Genomic structure of a double-stranded RNA virus in the parasite Leishmania major. In: Molecules and Cells. 1996 ; Vol. 6, No. 1. pp. 40-44.
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Suh, JM, Lee, GE, Kim, YM, Lee, JH, Kim, DM, Suh, JW & Chung, IK 1996, 'Genomic structure of a double-stranded RNA virus in the parasite Leishmania major', Molecules and Cells, vol. 6, no. 1, pp. 40-44.

Genomic structure of a double-stranded RNA virus in the parasite Leishmania major. / Suh, Jae Myoung; Lee, Gun Eui; Kim, Young Min; Lee, Joo Hun; Kim, Dae Myoung; Suh, Joo Won; Chung, In Kwon.

In: Molecules and Cells, Vol. 6, No. 1, 29.02.1996, p. 40-44.

Research output: Contribution to journalArticle

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Suh JM, Lee GE, Kim YM, Lee JH, Kim DM, Suh JW et al. Genomic structure of a double-stranded RNA virus in the parasite Leishmania major. Molecules and Cells. 1996 Feb 29;6(1):40-44.