Genomics, biological features, and biotechnological applications of Escherichia coli B: Is B for better?

Sung Ho Yoon, Haeyoung Jeong, Soon Kyeong Kwon, Jihyun F. Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Citations (Scopus)

Abstract

Strains of Escherichia coli B, especially BL21, have been widely used for overproducing recombinant proteins, ethanol, and other biomolecules. Almost all laboratory strains of E. coli are derivatives of non-pathogenic K-12 or B strains. While most genetic and metabolic studies have been performed with K-12 strains, little has been done on B strains. Recently, genome sequences of two E. coli strains of the B lineage, REL606 and BL21(DE3), have been determined, and results of multi-omics analyses were compared between B and K-12. As compared to K-12, B strains show a number of phenotypes such as faster growth in minimal media, lower acetate production, higher expression levels of recombinant proteins, and less degradation of such proteins during purification. In this review, we summarize the unique biological features of the B strains and overview their academic and industrial applications.

Original languageEnglish
Title of host publicationSystems Biology and Biotechnology of Escherichia coli
PublisherSpringer Netherlands
Pages1-17
Number of pages17
ISBN (Print)9781402093937
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology(all)

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    Yoon, S. H., Jeong, H., Kwon, S. K., & Kim, J. F. (2009). Genomics, biological features, and biotechnological applications of Escherichia coli B: Is B for better? In Systems Biology and Biotechnology of Escherichia coli (pp. 1-17). Springer Netherlands. https://doi.org/10.1007/978-1-4020-9394-4_1