Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea.

Sang Mee Guk, Tai Soon Yong, Soon Jung Park, Jae Hwan Park, Jong Yil Chai

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14 Citations (Scopus)

Abstract

Cryptosporidium parvum oocysts were isolated from a child suffering from acute gastroenteritis and successfully passaged in a calf and mice (designated hereafter SNU-H1) in the Republic of Korea; its molecular genotype has been analyzed. The GAG microsatellite region was amplified by a polymerase chain reaction (PCR), with a 238 base pair product, which is commonly displayed in C. parvum. The isolate was shown to be a mixture of the genotypes 1 (anthroponotic) and 2 (zoonotic). To study its infectivity in animals, 2 calves and 3 strains of mice were infected with the SNU-H1; in these animals, the propagation of both genotypes was successful. In immunosuppressed (ImSP) BALB/c and C57BL/6 mice the number of oocysts decreased after day 10 post-infection (PI); but in ImSP ICR mice, they remained constant until day 27 PI. The results show that both the C. parvum genotypes 1 and 2 can be propagated in calves and ImSP mice.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalThe Korean journal of parasitology
Volume42
Issue number2
DOIs
Publication statusPublished - 2004 Jun

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

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