Genotypes and genetic characters of Mycobacterium tuberculosis from Myanmar using three typing methods

Phyu Win Ei, Jong Seok Lee, Wah Wah Aung, Kim Yeun, Workneh Korma, Wint Wint Nyunt, Thyn Lei Swe, Mi Mi Htwe, Su Mon Win, Si Thu Aung, Chulhun L. Chang, Hyeyoung Lee

Research output: Contribution to journalArticle

Abstract

Knowledge on basic characteristics of Mycobacterium tuberculosis (MTB) is helpful to understand the disease epidemiology and support the prediction of clinical outcome of the disease. The aim of this study was to detect the genotypes and genotypic characters of clinical Mycobacterium tuberculosis (MTB) isolates from new and retreatment rifampicin-resistant patients using three different genotyping methods. Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) typing was used to determine the diversity of 222 clinical isolates. Spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) typing were also used to investigate the genetic characters of 105 MTB strains. Among the 15 genotypes detected by MIRU-VNTR, Beijing strains were the most prevalent of all strains (54.8%); new cases (40.5%) and retreatment cases (69.4%), followed by EAI strain. Spoligotyping categorized the strains into 11 lineages and 13 orphans whereas 96 different IS6110 patterns were identified using RFLP method. The mode number of IS6110 was 18 and 20. Higher band numbers were found in Beijing genotype (p < 0.001). Clustering rates by spoligotyping, MIRU-VNTR and IS6110-RFLP typing were 0.714, 0.004 and 0.085, respectively. Discriminatory powers of spoligotyping, MIRU-VNTR typing and IS6110-RFLP typing were 0.637, 1.000 and 0.997, respectively. Dominant Beijing genotype in both new and retreatment cases denoting that prevailing tuberculosis in Myanmar changed from EAI to Beijing lineage.

Original languageEnglish
Article number104005
JournalInfection, Genetics and Evolution
Volume75
DOIs
Publication statusPublished - 2019 Nov

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Myanmar
Minisatellite Repeats
tuberculosis
Mycobacterium tuberculosis
Restriction Fragment Length Polymorphisms
minisatellite repeats
Retreatment
polymorphism
genotype
Genotype
restriction fragment length polymorphism
China
orphan
Orphaned Children
Rifampin
epidemiology
methodology
rifampicin
Cluster Analysis
Epidemiology

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Ei, Phyu Win ; Lee, Jong Seok ; Aung, Wah Wah ; Yeun, Kim ; Korma, Workneh ; Nyunt, Wint Wint ; Swe, Thyn Lei ; Htwe, Mi Mi ; Win, Su Mon ; Aung, Si Thu ; Chang, Chulhun L. ; Lee, Hyeyoung. / Genotypes and genetic characters of Mycobacterium tuberculosis from Myanmar using three typing methods. In: Infection, Genetics and Evolution. 2019 ; Vol. 75.
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abstract = "Knowledge on basic characteristics of Mycobacterium tuberculosis (MTB) is helpful to understand the disease epidemiology and support the prediction of clinical outcome of the disease. The aim of this study was to detect the genotypes and genotypic characters of clinical Mycobacterium tuberculosis (MTB) isolates from new and retreatment rifampicin-resistant patients using three different genotyping methods. Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) typing was used to determine the diversity of 222 clinical isolates. Spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) typing were also used to investigate the genetic characters of 105 MTB strains. Among the 15 genotypes detected by MIRU-VNTR, Beijing strains were the most prevalent of all strains (54.8{\%}); new cases (40.5{\%}) and retreatment cases (69.4{\%}), followed by EAI strain. Spoligotyping categorized the strains into 11 lineages and 13 orphans whereas 96 different IS6110 patterns were identified using RFLP method. The mode number of IS6110 was 18 and 20. Higher band numbers were found in Beijing genotype (p < 0.001). Clustering rates by spoligotyping, MIRU-VNTR and IS6110-RFLP typing were 0.714, 0.004 and 0.085, respectively. Discriminatory powers of spoligotyping, MIRU-VNTR typing and IS6110-RFLP typing were 0.637, 1.000 and 0.997, respectively. Dominant Beijing genotype in both new and retreatment cases denoting that prevailing tuberculosis in Myanmar changed from EAI to Beijing lineage.",
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Ei, PW, Lee, JS, Aung, WW, Yeun, K, Korma, W, Nyunt, WW, Swe, TL, Htwe, MM, Win, SM, Aung, ST, Chang, CL & Lee, H 2019, 'Genotypes and genetic characters of Mycobacterium tuberculosis from Myanmar using three typing methods', Infection, Genetics and Evolution, vol. 75, 104005. https://doi.org/10.1016/j.meegid.2019.104005

Genotypes and genetic characters of Mycobacterium tuberculosis from Myanmar using three typing methods. / Ei, Phyu Win; Lee, Jong Seok; Aung, Wah Wah; Yeun, Kim; Korma, Workneh; Nyunt, Wint Wint; Swe, Thyn Lei; Htwe, Mi Mi; Win, Su Mon; Aung, Si Thu; Chang, Chulhun L.; Lee, Hyeyoung.

In: Infection, Genetics and Evolution, Vol. 75, 104005, 11.2019.

Research output: Contribution to journalArticle

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T1 - Genotypes and genetic characters of Mycobacterium tuberculosis from Myanmar using three typing methods

AU - Ei, Phyu Win

AU - Lee, Jong Seok

AU - Aung, Wah Wah

AU - Yeun, Kim

AU - Korma, Workneh

AU - Nyunt, Wint Wint

AU - Swe, Thyn Lei

AU - Htwe, Mi Mi

AU - Win, Su Mon

AU - Aung, Si Thu

AU - Chang, Chulhun L.

AU - Lee, Hyeyoung

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N2 - Knowledge on basic characteristics of Mycobacterium tuberculosis (MTB) is helpful to understand the disease epidemiology and support the prediction of clinical outcome of the disease. The aim of this study was to detect the genotypes and genotypic characters of clinical Mycobacterium tuberculosis (MTB) isolates from new and retreatment rifampicin-resistant patients using three different genotyping methods. Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) typing was used to determine the diversity of 222 clinical isolates. Spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) typing were also used to investigate the genetic characters of 105 MTB strains. Among the 15 genotypes detected by MIRU-VNTR, Beijing strains were the most prevalent of all strains (54.8%); new cases (40.5%) and retreatment cases (69.4%), followed by EAI strain. Spoligotyping categorized the strains into 11 lineages and 13 orphans whereas 96 different IS6110 patterns were identified using RFLP method. The mode number of IS6110 was 18 and 20. Higher band numbers were found in Beijing genotype (p < 0.001). Clustering rates by spoligotyping, MIRU-VNTR and IS6110-RFLP typing were 0.714, 0.004 and 0.085, respectively. Discriminatory powers of spoligotyping, MIRU-VNTR typing and IS6110-RFLP typing were 0.637, 1.000 and 0.997, respectively. Dominant Beijing genotype in both new and retreatment cases denoting that prevailing tuberculosis in Myanmar changed from EAI to Beijing lineage.

AB - Knowledge on basic characteristics of Mycobacterium tuberculosis (MTB) is helpful to understand the disease epidemiology and support the prediction of clinical outcome of the disease. The aim of this study was to detect the genotypes and genotypic characters of clinical Mycobacterium tuberculosis (MTB) isolates from new and retreatment rifampicin-resistant patients using three different genotyping methods. Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) typing was used to determine the diversity of 222 clinical isolates. Spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) typing were also used to investigate the genetic characters of 105 MTB strains. Among the 15 genotypes detected by MIRU-VNTR, Beijing strains were the most prevalent of all strains (54.8%); new cases (40.5%) and retreatment cases (69.4%), followed by EAI strain. Spoligotyping categorized the strains into 11 lineages and 13 orphans whereas 96 different IS6110 patterns were identified using RFLP method. The mode number of IS6110 was 18 and 20. Higher band numbers were found in Beijing genotype (p < 0.001). Clustering rates by spoligotyping, MIRU-VNTR and IS6110-RFLP typing were 0.714, 0.004 and 0.085, respectively. Discriminatory powers of spoligotyping, MIRU-VNTR typing and IS6110-RFLP typing were 0.637, 1.000 and 0.997, respectively. Dominant Beijing genotype in both new and retreatment cases denoting that prevailing tuberculosis in Myanmar changed from EAI to Beijing lineage.

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