Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences

T. S. Yong, S. J. Park, U. W. Hwang, H. W. Yang, K. W. Lee, D. Y. Min, H. J. Rim, Y. Wang, F. Zheng

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Genetic characterization of a total of 15 Giardia lamblia isolates, 8 from Anhui Province, China (all from purified cysts) and 7 from Seoul, Korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal DNA (eukaryotic 16S rDNA). Phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other Giardia isolates. The maximum parsimony method revealed that G. lamblia isolates from humans in China and Korea are divided into 2 major lineages, assemblages A and B. All 7 Korean isolates were grouped into assemblage A, whereas 4 Chinese isolates were grouped into assemblage A and 4 into assemblage B. Two Giardia microti isolates and 2 dog-derived Giardia isolates also grouped into assemblage B, whereas Giardia ardeae and Giardia muris were unique.

Original languageEnglish
Pages (from-to)887-891
Number of pages5
JournalJournal of Parasitology
Volume86
Issue number4
Publication statusPublished - 2000 Sep 4

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Giardia
Giardia lamblia
Korea
cyst
Ribosomal DNA
ribosomal DNA
genotyping
Korean Peninsula
China
nucleotide sequences
DNA
Giardia muris
polymerase chain reaction
amplification
axenic culture
Cysts
phylogenetics
Axenic Culture
Arvicolinae
dogs

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Yong, T. S. ; Park, S. J. ; Hwang, U. W. ; Yang, H. W. ; Lee, K. W. ; Min, D. Y. ; Rim, H. J. ; Wang, Y. ; Zheng, F. / Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences. In: Journal of Parasitology. 2000 ; Vol. 86, No. 4. pp. 887-891.
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Yong, TS, Park, SJ, Hwang, UW, Yang, HW, Lee, KW, Min, DY, Rim, HJ, Wang, Y & Zheng, F 2000, 'Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences', Journal of Parasitology, vol. 86, no. 4, pp. 887-891.

Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences. / Yong, T. S.; Park, S. J.; Hwang, U. W.; Yang, H. W.; Lee, K. W.; Min, D. Y.; Rim, H. J.; Wang, Y.; Zheng, F.

In: Journal of Parasitology, Vol. 86, No. 4, 04.09.2000, p. 887-891.

Research output: Contribution to journalArticle

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AU - Park, S. J.

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AU - Min, D. Y.

AU - Rim, H. J.

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AU - Zheng, F.

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AB - Genetic characterization of a total of 15 Giardia lamblia isolates, 8 from Anhui Province, China (all from purified cysts) and 7 from Seoul, Korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal DNA (eukaryotic 16S rDNA). Phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other Giardia isolates. The maximum parsimony method revealed that G. lamblia isolates from humans in China and Korea are divided into 2 major lineages, assemblages A and B. All 7 Korean isolates were grouped into assemblage A, whereas 4 Chinese isolates were grouped into assemblage A and 4 into assemblage B. Two Giardia microti isolates and 2 dog-derived Giardia isolates also grouped into assemblage B, whereas Giardia ardeae and Giardia muris were unique.

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