Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide

Jinhee Kim, Donghyun Kim, Meeree Kim, Hojeong Kwon, Taekwang Oh, Choonghwan Lee

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Abstract

In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with IC50 value of 3.0 μg/ml after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

Original languageEnglish
Pages (from-to)532-536
Number of pages5
JournalJournal of microbiology and biotechnology
Volume15
Issue number3
Publication statusPublished - 2005 Jun 1

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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