Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide

Jinhee Kim, Donghyun Kim, Meeree Kim, Ho Jeong Kwon, Taekwang Oh, Choonghwan Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with IC50 value of 3.0 μg/ml after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

Original languageEnglish
Pages (from-to)532-536
Number of pages5
JournalJournal of microbiology and biotechnology
Volume15
Issue number3
Publication statusPublished - 2005 Jun 1

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Etoposide
Caspases
Apoptosis
Caspase 3
Penicillium
Inhibitory Concentration 50
Chromatography
Leukemia
Down-Regulation
High Pressure Liquid Chromatography
2,5-dihydroxybenzyl alcohol

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Kim, Jinhee ; Kim, Donghyun ; Kim, Meeree ; Kwon, Ho Jeong ; Oh, Taekwang ; Lee, Choonghwan. / Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide. In: Journal of microbiology and biotechnology. 2005 ; Vol. 15, No. 3. pp. 532-536.
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Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide. / Kim, Jinhee; Kim, Donghyun; Kim, Meeree; Kwon, Ho Jeong; Oh, Taekwang; Lee, Choonghwan.

In: Journal of microbiology and biotechnology, Vol. 15, No. 3, 01.06.2005, p. 532-536.

Research output: Contribution to journalArticle

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