Abstract
Background: The German cockroach has been reported to act as an allergen that might be associated with a protease reaction in asthma. However, the molecular identities of the antigens in German cockroach extract (GCE) with protease activity and the protease-activated receptors (PARs) that are activated by GCE in human airway epithelial cells have not been characterized. Objective: We investigated the direct effect of GCE on Ca2+ signaling in human airway epithelial cells and the type of PARs activated by GCE. Methods: The Ca2+-sensitive dye Fura2 was used to determine intracellular Ca2+ concentration ([Ca2+]i) by means of spectrofluorometry. Results: GCE induced a baseline type of [Ca 2+]i oscillations in a dose-dependent manner. The oscillations persisted for long periods of time In the absence of Ca 2+ entry across the plasma membrane, suggesting that the observed [Ca2+]i increases were due to Ca2+ release from intracellular stores. Accordingly, after depleting endoplasmic reticulum Ca2+ with thapsigargin, an endoplasmic reticulum Ca2+ ATPase inhibitor, the GCE-mediated [Ca2+]i signals were abolished. Whereas desensitization of PAR-1, PAR-3, and PAR-4 had no effect on GCE-mediated Ca2+ mobilization, no GCE-mediated [Ca 2+]i increase was observed after desensitization of PAR-2. Conclusions: These results indicate that GCE has a direct effect on human airway epithelial cells, in particular generating [Ca2+]i oscillations through Ca2+ release from thapsigargin-sensitive Ca 2+ stores through activation of PAR-2.
| Original language | English |
|---|---|
| Pages (from-to) | 315-319 |
| Number of pages | 5 |
| Journal | Journal of Allergy and Clinical Immunology |
| Volume | 113 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2004 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
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German cockroach extract activates protease-activated receptor 2 in human airway epithelial cells. / Hong, Jeong Hee; Lee, Syng Ill; Kim, Kyu Earn; Yong, Taisoon; Seo, Jeong Taeg; Sohn, Myung Hyun; Shin, DongMin.
In: Journal of Allergy and Clinical Immunology, Vol. 113, No. 2, 01.01.2004, p. 315-319.Research output: Contribution to journal › Article
TY - JOUR
T1 - German cockroach extract activates protease-activated receptor 2 in human airway epithelial cells
AU - Hong, Jeong Hee
AU - Lee, Syng Ill
AU - Kim, Kyu Earn
AU - Yong, Taisoon
AU - Seo, Jeong Taeg
AU - Sohn, Myung Hyun
AU - Shin, DongMin
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Background: The German cockroach has been reported to act as an allergen that might be associated with a protease reaction in asthma. However, the molecular identities of the antigens in German cockroach extract (GCE) with protease activity and the protease-activated receptors (PARs) that are activated by GCE in human airway epithelial cells have not been characterized. Objective: We investigated the direct effect of GCE on Ca2+ signaling in human airway epithelial cells and the type of PARs activated by GCE. Methods: The Ca2+-sensitive dye Fura2 was used to determine intracellular Ca2+ concentration ([Ca2+]i) by means of spectrofluorometry. Results: GCE induced a baseline type of [Ca 2+]i oscillations in a dose-dependent manner. The oscillations persisted for long periods of time In the absence of Ca 2+ entry across the plasma membrane, suggesting that the observed [Ca2+]i increases were due to Ca2+ release from intracellular stores. Accordingly, after depleting endoplasmic reticulum Ca2+ with thapsigargin, an endoplasmic reticulum Ca2+ ATPase inhibitor, the GCE-mediated [Ca2+]i signals were abolished. Whereas desensitization of PAR-1, PAR-3, and PAR-4 had no effect on GCE-mediated Ca2+ mobilization, no GCE-mediated [Ca 2+]i increase was observed after desensitization of PAR-2. Conclusions: These results indicate that GCE has a direct effect on human airway epithelial cells, in particular generating [Ca2+]i oscillations through Ca2+ release from thapsigargin-sensitive Ca 2+ stores through activation of PAR-2.
AB - Background: The German cockroach has been reported to act as an allergen that might be associated with a protease reaction in asthma. However, the molecular identities of the antigens in German cockroach extract (GCE) with protease activity and the protease-activated receptors (PARs) that are activated by GCE in human airway epithelial cells have not been characterized. Objective: We investigated the direct effect of GCE on Ca2+ signaling in human airway epithelial cells and the type of PARs activated by GCE. Methods: The Ca2+-sensitive dye Fura2 was used to determine intracellular Ca2+ concentration ([Ca2+]i) by means of spectrofluorometry. Results: GCE induced a baseline type of [Ca 2+]i oscillations in a dose-dependent manner. The oscillations persisted for long periods of time In the absence of Ca 2+ entry across the plasma membrane, suggesting that the observed [Ca2+]i increases were due to Ca2+ release from intracellular stores. Accordingly, after depleting endoplasmic reticulum Ca2+ with thapsigargin, an endoplasmic reticulum Ca2+ ATPase inhibitor, the GCE-mediated [Ca2+]i signals were abolished. Whereas desensitization of PAR-1, PAR-3, and PAR-4 had no effect on GCE-mediated Ca2+ mobilization, no GCE-mediated [Ca 2+]i increase was observed after desensitization of PAR-2. Conclusions: These results indicate that GCE has a direct effect on human airway epithelial cells, in particular generating [Ca2+]i oscillations through Ca2+ release from thapsigargin-sensitive Ca 2+ stores through activation of PAR-2.
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U2 - 10.1016/j.jaci.2003.11.026
DO - 10.1016/j.jaci.2003.11.026
M3 - Article
VL - 113
SP - 315
EP - 319
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 2
ER -